Status of tumor infiltrating dendritic cells in glioma tissue and the expression levels of MHC-Ⅱ and CD54 in such cells

Objective To investigate the number of tumor infiltrating dendritic cells in glioma tissue and the expression levels of MHC-Ⅱ and CD54 in such cells. Methods Total 70 cases of glioma tissue specimens were taken as glioma tissue group, and 70 cases of normal brain tissue specimens were taken as normal brain tissue group in Ningbo Lihuili Hospital from January, 2013 to June, 2017. The glioma group was divided into low grade glioma group (grade Ⅰ + grade Ⅱ, 19 cases) and high grade glioma group (grade Ⅲ + Ⅳ, 51 cases) according to WHO pathological grade. The number of TIDCs in brain glioma tissues and the number of dendritic cells (DCs) in normal brain tissues were measured by immunohistochemistry. The expressions of MHC-Ⅱ and CD54 on the surface of DCs were detected by flow cytometry in TIDC and normal brain tissues. Results The nuclei appear as brown particles were S-100 positive staining DC cells. The number of TIDCs in glioma tissue was lower than that in normal brain tissue (P<0.05). The number of TIDCs in high-grade gliomas was lower than that in low-grade gliomas (P<0.05). The expression of MHC-Ⅱ and CD54 on the surface of TIBC was lower than that in normal brain tissue (P<0.05). The expression of MHC-Ⅱ and CD54 on the TIDC membrane in high-grade gliomas tissue was higher than that in the low-grade glioma tissue (P<0.05). Conclusion TIDC cells in brain glioma tissue is less than normal brain tissue DC cells. With the increase of glioma grade, TIDC cell infiltration decreases. The presence of antigenic presentation in the glioma is absent or decreased.