Expression and significance of IL-17 and IL-22 in tuberculosis heat-resistant antigen and phospho-antigen stimulated cell amplified polarization

SHENG Ling-ling; JIN hui; YANG Zai-xing; LI Bai-qing

doi:10.16766/j.cnki.issn.1674-4152.001881

Volume 19 Issue 4

Apr. 2021

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Article Navigation > Chinese Journal of General Practice > 2021 > 19(4): 636-638,697

SHENG Ling-ling, JIN hui, YANG Zai-xing, LI Bai-qing. Expression and significance of IL-17 and IL-22 in tuberculosis heat-resistant antigen and phospho-antigen stimulated cell amplified polarization[J]. Chinese Journal of General Practice, 2021, 19(4): 636-638,697. doi: 10.16766/j.cnki.issn.1674-4152.001881

Citation:

SHENG Ling-ling, JIN hui, YANG Zai-xing, LI Bai-qing. Expression and significance of IL-17 and IL-22 in tuberculosis heat-resistant antigen and phospho-antigen stimulated cell amplified polarization[J]. Chinese Journal of General Practice, 2021, 19(4): 636-638,697. doi: 10.16766/j.cnki.issn.1674-4152.001881

Citation:

SHENG Ling-ling, JIN hui, YANG Zai-xing, LI Bai-qing. Expression and significance of IL-17 and IL-22 in tuberculosis heat-resistant antigen and phospho-antigen stimulated cell amplified polarization[J]. Chinese Journal of General Practice, 2021, 19(4): 636-638,697. doi: 10.16766/j.cnki.issn.1674-4152.001881

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Expression and significance of IL-17 and IL-22 in tuberculosis heat-resistant antigen and phospho-antigen stimulated cell amplified polarization

doi: 10.16766/j.cnki.issn.1674-4152.001881

SHENG Ling-ling^{1, 2}
, JIN hui³
, YANG Zai-xing¹
, LI Bai-qing^{2
,
,}

1.
Department of Laboratory Medicine, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, China

Funds:

81072426

Received Date: 2020-04-09
Available Online: 2022-02-16

Abstract

Abstract

Objective To clarified the expression of IL-17 and IL-22 in tuberculosis heat-resistant antigen (Mtb-Hag) and phosphorylated antigen (HDMAPP) under the polarization state of IL-1β, TGF-β and IL-23 Happening. Methods Peripheral blood mononuclear cells (PBMC) were obtained by density gradient centrifugation and cultured in RPMI1640 medium. The experimental group was divided into three groups: Mtb-HAg, HDMAPP, and whole bacterial antigens. Each group was cultured with (IL-1β, TGF-β) for 3 days, rIL-2 for further culture, and IL-23 for 12 days on the 9th day. The control group was divided into three groups: Mtb-HAg, HDMAPP, and whole cell antigen. After 3 days of culture, rIL-2 was added to continue to culture until 12 days. The experimental group and the control group finally expanded into a cell population rich in effector γδ T cells, then the cells were collected and stimulated with PMA/Ionomycin and Monensin for 6 hours, and Tγδ 17 and Tγδ 22 were detected and analyzed. The proportion of cell subpopulations was compared and analyzed. Results In all groups, γδ T cells could produce IL-17 and IL-22. The percentage of IL-17 cells produced by γδ T cells in the Mtb-Hag+ck group was significantly higher than that in the Mtb-HAg group, and the HDMAPP+ck group was higher than the HDMAPP group (all P < 0.05). IL-17 was mainly produced by Vδ2 subpopulations, but there was no significant difference in the proportion of IL-22 cells produced by γ δ T cells in different polarization groups. Conclusion Under the polarization of IL-1 β, TGF-β and IL-23, the cultured PBMC stimulated by Mtb-Hag and HDMAPP can induce more IL-17 production by γδ T cells, but not IL-22.
- Tuberculosis,
- Mtb-HAg,
- HDMAPP,
- IL-17,
- IL-22

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References(21)

References

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