Volume 19 Issue 7
Jul.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(7): 1191-1195
LIU Xiu-jing, CHEN Hua-le, YU Jian, CHEN Zhan-guo. The value of a novel automatic nucleic acid system in detecting Epstein-Barr virus for the rapid diagnosis of infectious mononucleosis in children[J]. Chinese Journal of General Practice, 2021, 19(7): 1191-1195. doi: 10.16766/j.cnki.issn.1674-4152.002018
Citation: LIU Xiu-jing, CHEN Hua-le, YU Jian, CHEN Zhan-guo. The value of a novel automatic nucleic acid system in detecting Epstein-Barr virus for the rapid diagnosis of infectious mononucleosis in children[J]. Chinese Journal of General Practice, 2021, 19(7): 1191-1195. doi: 10.16766/j.cnki.issn.1674-4152.002018
shu

The value of a novel automatic nucleic acid system in detecting Epstein-Barr virus for the rapid diagnosis of infectious mononucleosis in children

doi: 10.16766/j.cnki.issn.1674-4152.002018

  • Department of Laboratory Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China

Funds:

 LGF20H200005

 Y20190473

  • Received Date: 2020-03-23
    Available Online: 2022-02-16
    Abstract
  •   Objective  To verify the performance of a novel automatic nucleic acid system in detecting Epstein-Barr virus (EBV) and evaluate its value in the rapid diagnosis of infectious mononucleosis (IM) in children.  Methods  The automatic EBV quantitative detection system involves nucleic acid extraction and quantitative detection via real-time fluorescence PCR. Whole blood samples were extracted using a PANA9600S automatic rotating nucleic acid workstation and amplified using an ABI7500 real-time fluorescence PCR. The main performance indexes of the EBV quantitative detection system, such as accuracy, precision, linear range, detection limit, antipollution ability, cross reaction and anti-interference ability, were evaluated. Finally, the detection system was used for nucleic acid quantitative detection of 50 children suspected with IM. The results of EBV nucleic acid detection were compared with those of other existing indicators.  Results  The EBV automatic quantitative detection system had good accuracy, and its high, medium and low levels of quantitative standards were within the range of fixed value. Its detection precision was high, and both CV values within and between batches were < 5%. The detection system was within the linear range of 1.0×103-1.0×108 copies/mL. The linear correlation coefficient was above 0.980, and the detection limit was 5.0×102 copies/mL. Moreover, the detection system had good antipollution ability, no cross reaction and had a strong anti-interference ability. EBV nucleic acid test was conducted on the peripheral blood of 50 children with suspected EBV infection. The positive detection rate was 80.0%, and the quantitative value of EBV ranged from 5.45×102 copies/mL to 1.46×108 copies/mL, which was significantly higher than the detection of serum EBV IgM antibody (P=0.031) and blood routine detection of abnormal lymphocyte ratio (P < 0.01).  Conclusion  The novel EBV automatic quantitative nucleic acid detection system described herein has good performance indexes and thus can meet the requirements of clinical detection. Therefore, it can be utilised for the rapid diagnosis of children with suspected IM. Thus, its clinical application must be promoted.

     

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