Volume 19 Issue 8
Aug.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(8): 1292-1295,1313
WANG Pan, WANG Yichao, YU Dandan, CHEN Wenju, ZHONG Qianyi, TONG Binxin. Expression of microRNA-1246 in the serum of hepatocellular carcinoma and its effect on biological function of HepG2 cells[J]. Chinese Journal of General Practice, 2021, 19(8): 1292-1295,1313. doi: 10.16766/j.cnki.issn.1674-4152.002043
Citation: WANG Pan, WANG Yichao, YU Dandan, CHEN Wenju, ZHONG Qianyi, TONG Binxin. Expression of microRNA-1246 in the serum of hepatocellular carcinoma and its effect on biological function of HepG2 cells[J]. Chinese Journal of General Practice, 2021, 19(8): 1292-1295,1313. doi: 10.16766/j.cnki.issn.1674-4152.002043
shu

Expression of microRNA-1246 in the serum of hepatocellular carcinoma and its effect on biological function of HepG2 cells

doi: 10.16766/j.cnki.issn.1674-4152.002043
  • 1.

    Department of Clinical Laboratory Medicine, Taizhou Central Hospital (Taizhou University Hospital), Institute of Molecular Diagnosis, Taizhou, Zhejiang 318000, China

Funds:

 2018C37037

 1901ky35

  • Received Date: 2020-08-06
    Available Online: 2022-02-16
    Abstract
  •   Objective  To investigate the expression of microRNA-1246 (miR-1246) in hepatocellular carcinoma (HCC) serum and its effect on the biological function of HepG2 cells.  Methods  Serum miR-1246 was determined by qRT-PCR in HCC patients and healthy controls. MiR-1246 mimics and NC mimics were transfected into HepG2 cells separately. The effect of miR-1246 on the biological function of HepG2 cells was examined by transwell chamber assay, scratch test and cell clone formation test. The expression of NFE2L3, Vimentin and GAS1 were detected by qRT-PCR.  Results  The expression levels of miR-1246 in the serum of healthy controls and HCC patients were 1.079 (0.740, 1.534) and 0.132 (0.068, 0.324), respectively, and the difference was statistically significant (P < 0.001). After transfection of miR-1246 mimics into HepG2 cells, the number of HepG2 cells passing through matrigel was (128.000±9.900), which was significantly lower than that of the NC mimic group (251.500±24.749, P=0.023). Forty-eight hours after scratch, the relative mobility of HepG2 cells was (24.833±3.351) %, which was significantly lower than that in the NC mimic group (31.859±7.194) % (P=0.046). The relative expression levels of Vimentin, GAS1 and NFE2L3 were (0.318±0.213), (0.479±0.157) and (0.755±0.044), respectively, which were significantly lower than those in the NC mimic group (P=0.046, P=0.043 and P=0.030, respectively).  Conclusion  The expression levels of miR-1246 were decreased in HCC serum. MiR-1246 could inhibit the invasion and migration of HepG2 cells by down-regulating the expression of Vimentin, GAS1 and NFE2L3.

     

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