Volume 19 Issue 11
Nov.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(11): 1831-1834
HAN Hong-liang, HONG Dao-xian, CHEN Ying, SONG Jun-jie, HE Dong-hai, LI Hong-ying, JIA Qiu-fang. Inhibition of trigeminal ganglion satellite glial cell inflammation by isohalothane regulating the NF-κB/iNOS-COX2 signalling pathway[J]. Chinese Journal of General Practice, 2021, 19(11): 1831-1834. doi: 10.16766/j.cnki.issn.1674-4152.002178
Citation: HAN Hong-liang, HONG Dao-xian, CHEN Ying, SONG Jun-jie, HE Dong-hai, LI Hong-ying, JIA Qiu-fang. Inhibition of trigeminal ganglion satellite glial cell inflammation by isohalothane regulating the NF-κB/iNOS-COX2 signalling pathway[J]. Chinese Journal of General Practice, 2021, 19(11): 1831-1834. doi: 10.16766/j.cnki.issn.1674-4152.002178
shu

Inhibition of trigeminal ganglion satellite glial cell inflammation by isohalothane regulating the NF-κB/iNOS-COX2 signalling pathway

doi: 10.16766/j.cnki.issn.1674-4152.002178
  • 1.

    Department of Anesthesiology, the First Affiliated Hospital of Henan University, Kaifeng, Henan 475000, China

Funds:

 18B310010

  • Received Date: 2020-05-15
    Available Online: 2022-02-15
    Abstract
  •   Objective  To investigate the effect of isohalothane (ISO) on rat trigeminal ganglion satellite glial cell inflammation in vitro and its mechanism.   Methods  Rat trigeminal ganglion satellite glial cells were cultured in vitro. Methyl thiazolyl tetrazolium and lactate dehydrogenase were used to select the appropriate ISO concentration. Cells were divided into the control group, model group, ISO group and SN50 group [nuclear factor (NF)-κB depressor] group. Nitroglycerin (GTN) was used to construct a satellite glial inflammatory model. The ISO group was treated with 1.5% ISO at a rate of 2 L/min for 30 min, and the other groups were treated with air at the same rate for 30 min. The levels of interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) and the content of NO in the cell culture medium were measured by enzyme-linked immunosorbent assay and nitric oxide (NO) assay. The protein expressions of NF-κB p65, cyclooxygenase-2 (COX2) and inducible nitric oxide synthase (iNOS) were detected by western blot. The concentration of calcium ions was detected by Fluo-3/AM probe loading method.   Results  The levels of IL-1β, TNF-α, PGE2 and NO and calcium ion concentration in the ISO group were (121.49±12.37) ng/L, (88.41±9.53) ng/L, (439.92±28.81) pg/mL, (9.03±1.04) μmol/L and (101.11±8.23) mmol/L, respectively, which were significantly lower than those in the model group (all P < 0.05). The results of western blotting analysis showed that the protein expression levels of NF-κB p65, COX2 and iNOS in the ISO group were 0.74±0.15, 0.59±0.14 and 0.51±0.11, respectively, which were significantly lower than those in the model group (all P < 0.05). The changing trend of each index in the SN50 group was consistent with that in the ISO group.   Conclusion  Appropriate dose of ISO can inhibit GTN induced inflammation of trigeminal ganglion satellite glial cells, and the mechanism may be related to reducing the release of inflammatory factors, inhibiting NF- κB/inos-COX2 signalling pathway and the decreasing the calcium concentration.

     

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