Volume 20 Issue 10
Oct.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(10): 1650-1653
JIANG Yu, SUN Xian-tao, SUN Shuang, LU Yue-bing. Effect of myocardial infarction-associated transcripts on retinoblastoma[J]. Chinese Journal of General Practice, 2022, 20(10): 1650-1653. doi: 10.16766/j.cnki.issn.1674-4152.002670
Citation: JIANG Yu, SUN Xian-tao, SUN Shuang, LU Yue-bing. Effect of myocardial infarction-associated transcripts on retinoblastoma[J]. Chinese Journal of General Practice, 2022, 20(10): 1650-1653. doi: 10.16766/j.cnki.issn.1674-4152.002670
shu

Effect of myocardial infarction-associated transcripts on retinoblastoma

doi: 10.16766/j.cnki.issn.1674-4152.002670

  • Department of Ophthalmology, Children' s Hospital of Zhengzhou University, Henan Children' s Hospital, Zhengzhou Children' s Hospital, Zhengzhou, Henan 450000, China

Funds:

 LHGJ20190952

  • Received Date: 2021-11-15
    Available Online: 2022-11-30
    Abstract
  •   Objective   To explores the effect of lncRNA MIAT targeting miR-145 on the proliferation and apoptosis of retinoblastoma.   Methods   From September 2015 to September 2020, 30 children with retinoblastoma underwent enucleation in Children ' s Hospital of Zhengzhou University were selected, and tumor tissues and adjacent tissues were collected. Human retinoblastoma cells (Y79) were cultured and divided into si-NC, si-MIAT, miR-NC, miR-145, si-MIAT + anti-miR-NC and si-MIAT + anti-miR-145 groups according to the random number table method. RT-qPCR was used in detecting the expression levels of MIAT and miR-145 in the cells. CCK-8 was used in detecting cell proliferation, and flow cytometry was used in detecting cell apoptosis. Cyclin D1, p21, Bcl-2, Bax, p-PI3K and p-Akt protein expression levels were detected with Western blotting, and dual luciferase reporter was used in detecting the targeted relationship between MIAT and miR-145.   Results   Compared with adjacent tissues, the expression of MIAT in retinoblastoma tissue was significantly up-regulated, and the expression of miR-145 was significantly down-regulated (3.61±0.28 vs. 1.05±0.11, 0.33±0.03 vs. 1.03±0.09, all P < 0.05). Compared with the si-NC group, cell viability in the si-MIAT group was significantly down-regulated, mortality rate was significantly up-regulated, and p-PI3K protein and p-Akt protein expression were significantly down-regulated (all P < 0.05). Compared with the miR-NC group, cell viability in the miR-145 group showed significantly down-regulated, apoptosis rate was significantly up-regulated (all P < 0.05). Compared with the si-MIAT + anti-miR-NC group, cell viability was significantly up-regulated in the si-MIAT + anti-miR-145 group, cell apoptosis rate was significantly down-regulated, whereas the expression levels of p-PI3K and p-Akt proteins were significantly up-regulated (all P < 0.05).   Conclusion   The inhibition of MIAT expression and up-regulation of miR-145 expression can promote apoptosis and inhibit the proliferation of retinoblastoma. The mechanism is related to the PI3K/Akt pathway.

     

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