Volume 21 Issue 2
Feb.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(2): 263-266
LI Dan-ping, LI Zong-min, ZHANG Li-xia, YANG Wei-wei, LI Qiao-li. Diagnostic value of serum miR-18a and miR-485-5p for HPV-positive cervical cancer[J]. Chinese Journal of General Practice, 2023, 21(2): 263-266. doi: 10.16766/j.cnki.issn.1674-4152.002860
Citation: LI Dan-ping, LI Zong-min, ZHANG Li-xia, YANG Wei-wei, LI Qiao-li. Diagnostic value of serum miR-18a and miR-485-5p for HPV-positive cervical cancer[J]. Chinese Journal of General Practice, 2023, 21(2): 263-266. doi: 10.16766/j.cnki.issn.1674-4152.002860
shu

Diagnostic value of serum miR-18a and miR-485-5p for HPV-positive cervical cancer

doi: 10.16766/j.cnki.issn.1674-4152.002860
  • 1.

    Department of Gynaecology, Yellow River Sanmenxia Hospital, Sanmenxia, Henan 472000, China

Funds:

 182102510069

  • Received Date: 2022-01-29
    Available Online: 2023-04-20
    Abstract
  •   Objective  To explore the difference of serum MicroRNA (miR)-18a and miR-485-5p expression between cervical cancer patients with human papillomavirus (HPV) and simple cervical cancer patients, and analyze the diagnostic value of the two indicators for HPV-positive cervical cancer.  Methods  A total of 91 patients with HPV-positive cervical cancer admitted to Yellow River Sanmenxia Hospital from January 2020 to January 2021 were selected as the HPV-positive cervical cancer group. Sixty-two women with HPV-positive non-cervical cancer were placed in the HPV-positive group, and 57 healthy women based on physical examination were placed in the control group. Serum miR-18a and miR-485-5p expression was detected by qRT-PCR. Receiver operating characteristic (ROC) curve was used to analyse the diagnostic value of two indicators for HPV-positive cervical cancer.  Results  The expression of serum miR-18a in HPV-positive cervical cancer group was higher, while the expression of miR-485-5p was lower than those in the control group and HPV-positive cervical cancer group (P < 0.05). Serum miR-18a and miR-485-5p expression was associated with FIGO stage, depth of interstitial infiltration and lymph node metastasis in patients with HPV-positive cervical cancer (P < 0.05). The ROC curves showed that the area under the curve (AUC) for miR-18a and miR-485-5p and their combination for the diagnosis of HPV-positive cervical cancer were 0.752, 0.802 and 0.876, respectively, with sensitivities of 67.03%, 75.82% and 83.52%, and specificities of 77.42%, 74.19% and 83.87%, respectively. The AUC of the combined diagnosis of HPV-positive cervical cancer was greater than that of miR-18a and miR-485-5p alone (P < 0.05).  Conclusion  High expression of serum miR-18a and low expression of miR-485-5p in HPV-positive cervical cancer can be used as an auxiliary diagnostic indicator for HPV-positive cervical cancer.

     

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