Volume 21 Issue 6
Jun.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(6): 936-939
HAN Yong, LIU Xinwen, WANG Chaohai, YANG Xinwei, LI Wenhua, JING Jiang. Protective effect of Xuesaitong capsule on myocardial cell hypoxia injury by inhibiting the expression of inflammatory factors[J]. Chinese Journal of General Practice, 2023, 21(6): 936-939. doi: 10.16766/j.cnki.issn.1674-4152.003019
Citation: HAN Yong, LIU Xinwen, WANG Chaohai, YANG Xinwei, LI Wenhua, JING Jiang. Protective effect of Xuesaitong capsule on myocardial cell hypoxia injury by inhibiting the expression of inflammatory factors[J]. Chinese Journal of General Practice, 2023, 21(6): 936-939. doi: 10.16766/j.cnki.issn.1674-4152.003019
shu

Protective effect of Xuesaitong capsule on myocardial cell hypoxia injury by inhibiting the expression of inflammatory factors

doi: 10.16766/j.cnki.issn.1674-4152.003019
  • 1.

    Department of Pharmacy, Affiliated Hospital of Shaoxing University of Arts and Sciences, Shaoxing, Zhejiang 312000, China

Funds:

 Y202146979

 2022KY066

  • Received Date: 2022-12-18
    Available Online: 2023-08-26
    Abstract
  •   Objective  To explore the protective effect of Xuesaitong capsule on oxidative damage of myocardial cells by establishing a mouse model of myocardial injury and treating the injured myocardial cells with Xuesaitong capsule.  Methods  A total of 25 SD rats were randomly divided into blank control group, myocardial cell hypoxia/reoxygenation (H/R) model group, low, medium and high dose groups of Xuesaitong capsule by rasom number table method, with 5 rats in each group. The myocardial cells of each group were cultured. The apoptosis rate of myocardial cells in each group were detected by flow cytometry. The inflammatory factors [interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α)] and oxidative stress indicators [lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD)] in each group were measured by ELISA, and NF-κB expression were detected by Western blotting.  Results  The apoptosis rate of myocardial cells in H/R group [(22.85±1.78)%] were higher than control group [(3.45±0.42)%] and the low, medium and high dose groups of Xuesaitong capsule [(10.25±2.02)%, (8.96±1.23)%, (5.88±1.45)%]. The serum IL-6, TNF-α, LDH, MDA and NF-κB in H/R group were significantly higher than control group and low, medium and high dose groups of Xuesaitong capsule (P < 0.05), while the activity of SOD in H/R group were significantly lower than that of control group and low, medium and high dose groups of Xuesaitong capsule (all P < 0.05).  Conclusion  Xuesaitong capsule has protective effect on H/R injury of myocardial cells, and its protective mechanism may be related to its inhibition of NF-κB gene overexpression.

     

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