Volume 22 Issue 1
Jan.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(1): 59-63
YIN Hezhai, SHEN Lidong, YUAN Mei, WANG Qi, HONG Zhonghua. Analysis of the difference of intestinal flora between patients with perianal abscess before and after surgery and healthy people[J]. Chinese Journal of General Practice, 2024, 22(1): 59-63. doi: 10.16766/j.cnki.issn.1674-4152.003331
Citation: YIN Hezhai, SHEN Lidong, YUAN Mei, WANG Qi, HONG Zhonghua. Analysis of the difference of intestinal flora between patients with perianal abscess before and after surgery and healthy people[J]. Chinese Journal of General Practice, 2024, 22(1): 59-63. doi: 10.16766/j.cnki.issn.1674-4152.003331
shu

Analysis of the difference of intestinal flora between patients with perianal abscess before and after surgery and healthy people

doi: 10.16766/j.cnki.issn.1674-4152.003331
  • 1.

    Department of Anorectal, Jiaxing Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medicine University, Jiaxing, Zhejiang 314001, China

Funds:

 LGF22H270005

 2022KY392

 2021ZB287

 2022ZB349

 2021AD30136

 2022AD30111

 2022AD10012

  • Received Date: 2023-01-19
    Available Online: 2024-03-09
    Abstract
  •   Objective  The intestinal flora distribution of perianal abscess patients before and after surgery was detected by 16S rDNA gene sequencing to explore whether the flora distribution was different from that of healthy people.  Methods  Clinical data of 15 patients with perianal abscess (observation group) and 15 healthy individuals (control group) who visited the Department of Anorectal of Jiaxing Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medicine University between August and November 2022 were collected, their fecal DNA was extracted, and their data including alpha diversity, beta diversity were analyzed by PCR amplification and 16S rDNA gene sequencing, and possible major hallmark flora of perianal abscess were screened.  Results  Alpha diversity analysis showed that compared to the control group, the observation group had preoperative levels of Chao1 and Observed_species increased, while the postoperative Shannon and Simpson indices decreased, with significant differences (P < 0.05). Compared with operative, postoperative Chao1, Shannon, Simpson, Observed_species in the observation decreased (P < 0.05). The relative abundance of Bacillota, Pseudomonadota, Actinomycetota and Bacteroidota was significantly different between the control group and the observation group before and after surgery (P < 0.05). Compared with the control group, the relative abundance of Actinomycetota in the observation group before surgery was reduced (P < 0.05), and the relative abundance of Pseudomonadota after surgery was increased (P < 0.05). Compared with operative, the relative abundance of Pseudomonadota in the observation increased after operation (P < 0.05). The main marker flora of perianal abscess was screened, and receiver operating characteristic analysis showed that Bilophila wadsworthia had the max AUC (AUC=0.831).  Conclusion  The intestinal flora of patients with perianal abscess differs significantly from that of healthy individuals before and after surgery, and the abundance of intestinal flora changes significantly after surgical treatment. Bilophila may be a major marker of perianal abscess.

     

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