Influence of neoadjuvant chemotherapy of TAC on curative effect,cellular immunity and Ki-67 expression in early breast cancer patients

Objective Neoadjuvant chemotherapy can improve the breast cancer patients'survival,but its immunological mechanisms was not clear,the purpose of this paper was study the influence of neoadjuvant chemotherapy of TAC on cellular immunity and Ki-67 expression in early breast cancer patients. Methods Ninety patients with early breast cancer patients were divided into control group 45 cases and treatment group 45 cases according to random number table method.The control group was used by CEF regimen,and the treatment group was used by TAC methods,3 weeks/courses,and a total 4 courses.The clinical efficacy and adverse reactions of two groups of patients were observed.The level of CD3,CD4,CD8 and natural killer cells (NK) of whole blood were tested by flow cytometry.The level of tumor necrosis factor alpha (TNF-α),interleukin 2(IL-2),interleukin 6(IL-6) and interleukin 10(IL-10) were detected by ELISA.The expression of Ki-67 was examined by immunohistochemistry. Results The total effective rate 86.67%(39/45) in treatment group was higher than that of control group 68.89%(31/45),P<0.05.After treatment the level of CD3,CD4,CD8 and NK in the experimental group were significantly lower than before treatment,and the the level of CD3,CD4,CD8 were lower than the control group (P<0.05).The level of TNF-α and IL-2 in the treatment group were significantly higher than the control group,and IL-6 and IL-10 were significantly lower than that of control group (P<0.05).After treatment,the positive rate of Ki-67 53.33%(24/45) and 57.78%(26/45) were significantly lower than before treatment 88.89%(40/45) and 84.44(38/45),χ²=0.997,0.402,allP<0.01,but no difference between the two groups (χ²=0.180,P=0.671).The incidence of adverse reaction 37.78(17/45) in the treatment group was lower than the control group 60.00%(27/45),χ²=4.447,P=0.035. Conclusion Neoadjuvant chemotherapy TAC scheme can improve the cellular immune function in patients with early breast cancer,reduce the positive expression of Ki-67,has a good clinical curative effect.