Effects of taurine on endoplasmic reticulum stress in rats with acute stress skeletal muscle injury

Objective To study the effect of taurine on GRP78, MAD, GADD153 and SOD in rats with eccentric exercise, in order to explain the mechanism of taurine protecting skeletal muscle injury by reducing endoplasmic reticulum oxidative stress. Methods A total of 36 adult SD rats were randomly divided into blank control 1 group(group A), blank control 2 group(group B), 1 day eccentric exercise group(group C), 2 days eccentric exercise group(group D), 1 day eccentric exercise+taurine group(group E), 2 days eccentric exercise+taurine group(group F). The skeletal muscle of 6 groups rats were collected, and the contents of SOD, MAD, GRP78 and GADD153 were measured. The content of SOD and MAD were measured by xanthine oxidase method and thiobarbituric acid method, respectively. The expressions of GRP78 and GADD153 were measured by Western blotting method. The differences of SOD, MAD, GRP78 and GADD153 among the 6 groups were compared. Results The SOD in group A and group B was higher than that in group C, group D, group E and group F, there was significant statistical difference compared with group C, group D(P<0.05), but there was no difference compared with group E and group F(P>0.05). The SOD in group E and group F was higher than that in group C and group D, there was no statistically significant difference(P>0.05). The MDA in group C and group D was higher than that in group A, group B, group E and group F(P<0.05). The MDA/SOD value in group A, group B, group E and group F was greater than group C and group D(P<0.05). The MDA/SOD value in group E and group F was lower than that in group A and group B(P<0.05). GRP78, GADD153 expression in group C and group D were higher than that in group A and group B(P<0.05), but was lower than that in group E and group F(P<0.05). GRP78, GADD153 expression in group E and group F were higher than that in group A and group B(P<0.05). Conclusion Taurine may be maintain the antioxidant capacity of skeletal muscle by improving the ability of endoplasmic reticulum oxidative stress, which can accelerate the elimination of oxygen free radicals in skeletal muscle and decrease oxidative stress in skeletal muscle after strenuous exercise. It is conducive to the recovery of skeletal muscle function.