Extraction and identification of exosomes from human nasopharyngeal carcinoma cell lines with different metastatic habits

GUO Shu-nan; WANG Geng-ming; ZHANG Lei; YING Qiao-ling; DING Jian-ming; ZHOU Yan; ZHOU Yu-fu; JIANG Hao

doi:10.16766/j.cnki.issn.1674-4152.001484

Volume 18 Issue 8

Aug. 2022

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Article Navigation > Chinese Journal of General Practice > 2020 > 18(8): 1276-1278,1340

GUO Shu-nan, WANG Geng-ming, ZHANG Lei, YING Qiao-ling, DING Jian-ming, ZHOU Yan, ZHOU Yu-fu, JIANG Hao. Extraction and identification of exosomes from human nasopharyngeal carcinoma cell lines with different metastatic habits[J]. Chinese Journal of General Practice, 2020, 18(8): 1276-1278,1340. doi: 10.16766/j.cnki.issn.1674-4152.001484

Citation:

GUO Shu-nan, WANG Geng-ming, ZHANG Lei, YING Qiao-ling, DING Jian-ming, ZHOU Yan, ZHOU Yu-fu, JIANG Hao. Extraction and identification of exosomes from human nasopharyngeal carcinoma cell lines with different metastatic habits[J]. Chinese Journal of General Practice, 2020, 18(8): 1276-1278,1340. doi: 10.16766/j.cnki.issn.1674-4152.001484

Citation:

GUO Shu-nan, WANG Geng-ming, ZHANG Lei, YING Qiao-ling, DING Jian-ming, ZHOU Yan, ZHOU Yu-fu, JIANG Hao. Extraction and identification of exosomes from human nasopharyngeal carcinoma cell lines with different metastatic habits[J]. Chinese Journal of General Practice, 2020, 18(8): 1276-1278,1340. doi: 10.16766/j.cnki.issn.1674-4152.001484

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Extraction and identification of exosomes from human nasopharyngeal carcinoma cell lines with different metastatic habits

doi: 10.16766/j.cnki.issn.1674-4152.001484

GUO Shu-nan¹
, WANG Geng-ming^{1,2
,
,}
, ZHANG Lei³
, YING Qiao-ling^1,2
, DING Jian-ming^1,2
, ZHOU Yan^1,2
, ZHOU Yu-fu^1,2
, JIANG Hao^1,2

Department of Radiation Oncology,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China

Received Date: 2019-10-14
Available Online: 2022-08-06

Abstract

Abstract

Objective To explore the expression of exosomes in human nasopharyngeal carcinoma(NPC) cell lines SUNE-1-5-8 F, SUNE-1-6-10 B, SUNE-1 and normal nasopharyngeal epithelial cells NP69, and to provide a theoretical basis for further study of new methods of diagnosis and treatment of NPC. Methods Exosomes were isolated from SUNE-1-5-8 F, SUNE-1-6-10 B, SUNE-1 and NP69 culture supernatants using exosome extraction kits, and verified by transmission electron microscopy. The concentration of exosome protein was determined by BCA kit. Eexosome specific antigen molecule CD63 and Flotillin-1 were detected by western bolt. Results Vesicles were isolated from supernatant of three kinds of nasopharyngeal carcinoma cell lines and normal nasopharyngeal epithelial cells, showed a circular vesicle under a transmission electron microscope. The exosomal protein concentrations of SUNE-1-5-8 F, SUNE-1-6-10 B, SUNE-1 and NP69 were 0.778, 0.635, 0.788, and 0.576 μg/μL, respectively. CD63 and Flotillin-1 were expressed in all cell lineage-derived exosomes. Conclusion Three kinds of nasopharyngeal carcinoma cell lines SUNE-1-5-8 F, SUNE-1-6-10 B, SUNE-1 and normal nasopharyngeal epithelial cell line NP69 all produce exosomes.
- Nasopharyngeal carcinoma,
- Exosomes,
- Identification