Volume 19 Issue 5
May  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(5): 736-739
CHU Na, ZHANG Xuan, RUAN Da-peng, CHEN Si-yuan, WANG Yun. Effect of luteolin on proliferation and migration of human lung cancer cell line H460[J]. Chinese Journal of General Practice, 2021, 19(5): 736-739. doi: 10.16766/j.cnki.issn.1674-4152.001904
Citation: CHU Na, ZHANG Xuan, RUAN Da-peng, CHEN Si-yuan, WANG Yun. Effect of luteolin on proliferation and migration of human lung cancer cell line H460[J]. Chinese Journal of General Practice, 2021, 19(5): 736-739. doi: 10.16766/j.cnki.issn.1674-4152.001904
shu

Effect of luteolin on proliferation and migration of human lung cancer cell line H460

doi: 10.16766/j.cnki.issn.1674-4152.001904

  • School of Public Health, Bengbu Medical College, Bengbu, Anhui 233000, China

Funds:

 KJ2019A0316

 Byycx1907

 Byycx20051

  • Received Date: 2020-11-26
    Available Online: 2022-02-16
    Abstract
  •   Objective  To explore the effect of luteolin (LUT) on the proliferation and migration of human lung large cell carcinoma H460 cells and analyse its mechanism of action.   Methods  After the H460 cells were treated with different concentrations of LUT for 24 h or 48 h, the cell viability was detected by MTT assay. The clone formation ability and migration ability were detected by plate clone formation test and scratch test, respectively. The expression levels of E-cadherin and N-cadherin protein were detected by Western blotting.   Results  LUT can inhibit the activity of H460 cells in a concentration-dependent and time-dependent manner (P < 0.05). After H460 cells were treated with 0, 10 and 20 μmol/L LUT for 48 h, the clone formation rates of the control, LUT10 and LUT20 groups respectively were 1.00±0.05, 0.78±0.05 and 0.49±0.04, and the scratch healing degree respectively were (30.37 ±2.92)%, (26.23±2.42)% and (22.16 ±1.74)%. The expression levels of N-cadherin and E-cadherin proteins were 0.62±0.01, 0.61±0.01 and 0.59±0.01; 0.85±0.05, 0.88 ±0.05 and 0.94 ±0.05, respectively. Compared with those of the control group, the plate clone formation rate and scratch healing rate of LUT10 and LUT20 groups decreased (all P < 0.05), the expression level of E-cadherin protein was up-regulated (all P < 0.05) and the expression level of N-cadherin protein was down-regulated (all P < 0.05).   Conclusion  LUT can effectively inhibit the proliferation and migration of H460 cells, which may be related to the regulation of E-cadherin and N-cadherin-related marker proteins of epithelial-mesenchymal transition.

     

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