Volume 19 Issue 6
Jun.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(6): 921-924
ZHANG Wen-ting, GU Lin, KE Xi-quan, WANG Qi-zhi, WANG Qiang-wu, WANG Jian-chao, LI Da-peng. Expressions of TLR9 and STAT3 and their correlation with IL-10 in patients with ulcerative colitis[J]. Chinese Journal of General Practice, 2021, 19(6): 921-924. doi: 10.16766/j.cnki.issn.1674-4152.001950
Citation: ZHANG Wen-ting, GU Lin, KE Xi-quan, WANG Qi-zhi, WANG Qiang-wu, WANG Jian-chao, LI Da-peng. Expressions of TLR9 and STAT3 and their correlation with IL-10 in patients with ulcerative colitis[J]. Chinese Journal of General Practice, 2021, 19(6): 921-924. doi: 10.16766/j.cnki.issn.1674-4152.001950
shu

Expressions of TLR9 and STAT3 and their correlation with IL-10 in patients with ulcerative colitis

doi: 10.16766/j.cnki.issn.1674-4152.001950

  • Department of Gastroenterology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 gxgwfx2018052

 BYKY2019085ZD

  • Received Date: 2020-10-22
    Available Online: 2022-02-16
    Abstract
  •   Objective  The expression levels of toll-like receptor 9 (TLR9), signal transduction and transcriptional activator factor 3 (STAT3) and interleukin-10 (IL-10) in peripheral blood and intestinal mucosal tissues of patients with ulcerative colitis (UC) were detected to explore their roles in the pathogenesis of UC and their correlation.  Methods  Thirty-two patients with UC treated in the First Affiliated Hospital of Bengbu Medical College from July 2019 to July 2020 were selected as the experimental group; and 30 healthy subjects according to the results of physical examination were included in the normal control group. The experimental group was further divided into mild group (n=10), moderate group (n=14) and severe (n=8) group. The expressions of TLR9 and STAT3 mRNA in peripheral blood mononuclear cells, serum IL-10 levels and TLR9, as well as levels of STAT3 and IL-10 proteins, in intestinal mucosal tissues were detected.  Results  The expression levels of TLR9 mRNA and STAT3 mRNA in the experimental group were both higher than those in the control group (all P < 0.05). The expression level of IL-10 in serum in the experimental group was significantly lower than that in the normal control group (P < 0.05). The expression levels of TLR9 and STAT3 in intestinal mucosal tissues were all higher than that in the normal control group (all P < 0.05). By contrast, the expression levels of IL-10 in the experimental group were lower than that in the normal control group (P < 0.05). Expressions of TLR9 mRNA and STAT3 mRNA and TLR9 and STAT3 mRNA were positively correlated. Serum IL-10 was negatively correlated with TLR9 and STAT3 mRNA expression levels. Intestinal mucosal IL-10 was negatively correlated with TLR9 and STAT3 protein expression levels.  Conclusion  TLR9, STAT3 and IL-10 are jointly involved in the occurrence and development of UC. TLR9 and STAT3 may be involved in the expression and regulation of IL-10 in the pathogenesis of UC.

     

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