Volume 19 Issue 8
Aug.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(8): 1283-1286
ZHAO Ping, LI Xin, CUI Jing, XIA Xiaoshuang, TIAN Wenjie. Effect of Ginkgo biloba extract on bFGF/Notch1 pathway in hippocampus of rats models with Alzheimer's disease[J]. Chinese Journal of General Practice, 2021, 19(8): 1283-1286. doi: 10.16766/j.cnki.issn.1674-4152.002041
Citation: ZHAO Ping, LI Xin, CUI Jing, XIA Xiaoshuang, TIAN Wenjie. Effect of Ginkgo biloba extract on bFGF/Notch1 pathway in hippocampus of rats models with Alzheimer's disease[J]. Chinese Journal of General Practice, 2021, 19(8): 1283-1286. doi: 10.16766/j.cnki.issn.1674-4152.002041
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Effect of Ginkgo biloba extract on bFGF/Notch1 pathway in hippocampus of rats models with Alzheimer's disease

doi: 10.16766/j.cnki.issn.1674-4152.002041
  • 1.

    Department of Neurology, the Second Hospital of Tianjin Medical University, Tianjin 300211, China

Funds:

 2017162

  • Received Date: 2020-04-20
    Available Online: 2022-02-16
    Abstract
  •   Objective  To explore the mechanism of Ginkgo biloba extract (GBE) in repairing AD rats' neurons related to Aβ25-35.  Methods  According to the random method, 60 rats meeting the requirements were divided into model group, normal control group, sham operation group, Ginkgo biloba experimental group (high, medium, low), 10 rats in each group. Model was built by intrathecal injection of Aβ25-35. All rats were studied by testing the memory and learning abilities were tested by Morris water maze. The expression levels of RBP-JK, Hes1, bFGF and Notch1 protein in hippocampus were detected by immunoblotting method.  Results  The time of finding platform in the AD model group [(60.190±3.821) s] was significantly longer than that in the normal control group (P < 0.01), and the frequency of finding the original platform position (4.310±2.057) was significantly reduced (P < 0.01). Compared with the model group, the time of finding the platform for the first time in the Ginkgo biloba high, middle and low dose groups were significantly reduced (P < 0.05), and the number of crossing platform was significantly more than that in the model group (all P < 0.01). The expression of bFGF (0.542±0.036), Notch1 (0.185±0.032) and Hes1 (0.153±0.022) in the model group were significantly decreased, while RBP-JK protein expression (0.800±0.047) was significantly increased (all P < 0.01). The protein expression of bFGF, Notch1 and Hes1 in the hippocampus of the Ginkgo biloba extract high, middle and low group were higher than that in the model group (all P < 0.05), while the protein expression of RBP-JK was decreased (all P < 0.05).  Conclusion  Ginkgo biloba extract may promote the expression of Notch1, bFGF and Hes1 and inhibit the expression of RBP-JK by activating the Notch pathway, thereby improving the learning ability and memory ability of AD rats.

     

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