Volume 19 Issue 9
Sep.  2021
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ZHANG Bing, XIE Jing-ri, SUN Tao. Effects of Changyuning Decoction on protein expression of claudin-1, ZO-1 and occludin in colonic tissue of rats with ulcerative colitis[J]. Chinese Journal of General Practice, 2021, 19(9): 1447-1450, 1577. doi: 10.16766/j.cnki.issn.1674-4152.002082
Citation: ZHANG Bing, XIE Jing-ri, SUN Tao. Effects of Changyuning Decoction on protein expression of claudin-1, ZO-1 and occludin in colonic tissue of rats with ulcerative colitis[J]. Chinese Journal of General Practice, 2021, 19(9): 1447-1450, 1577. doi: 10.16766/j.cnki.issn.1674-4152.002082

Effects of Changyuning Decoction on protein expression of claudin-1, ZO-1 and occludin in colonic tissue of rats with ulcerative colitis

doi: 10.16766/j.cnki.issn.1674-4152.002082
Funds:

 81904152

 2017M611410

 2021ZQ073

  • Received Date: 2020-02-19
    Available Online: 2022-02-15
  •   Objective  To investigate the effect of Changyuning Decoction on the expression of key proteins occludin, claudin-1 and zona occludens-1 (ZO-1) in the tight junction (TJ) of colonic tissue of model rats with ulcerative colitis (UC).   Methods  Sixty healthy male SD rats were randomly divided into the blank control group, model control group, Changyuning group and mesalazine group, with 15 rats in each group. The UC rat model was prepared by single enema with 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)/ethanol complex solution (100 mg/kg TNBS+0.25 mL of 50% ethanol). After successful modelling, drug intervention was performed for 7 days. A light microscope was used to observe the pathomorphological changes of rat colon tissues, a transmission electron microscope was employed to observe the ultrastructure of rat colon mucosa, and immunofluorescence staining was utilised to determine the fluorescence intensities of occludin, claudin-1 and ZO-1 proteins in rat colon tissues.   Results  Light and electron microscopic observations showed that the colonic mucosa of UC model rats was congested and oedematous, with obvious inflammatory exudation and broken intercellular TJs. After Changyuning intervention, the pathological status of the colonic mucosa of rats was significantly improved, and a small part of intercellular TJs was broken. The results of immunofluorescence staining showed that the fluorescence intensities of claudin-1, ZO-1 and occludin protein in the colon tissue of UC model rats (0.11±0.01, 0.11±0.01, 0.09±0.01) were significantly lower than those in the blank control group (0.14±0.01, 0.15±0.03, 0.13±0.00, all P < 0.05). After Changyuning intervention, the fluorescence intensities of claudin-1, ZO-1 and occludin in the colon tissue of rats were significantly higher than those in the model control group (0.13±0.00, 0.14±0.02, 0.12±0.01, all P < 0.05).   Conclusion  Changyuning Decoction can repair colonic mucosal injury to treat UC by up-regulating the expression of key proteins of TJ including claudin-1, ZO-1 and occludin.

     

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