Volume 19 Issue 9
Sep.  2021
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Article Navigation >  Chinese Journal of General Practice  > 2021  >  19(9): 1578-1583
CHEN Zhi-neng, XIE Li-li, CHEN Hua, YAN Kun, LI Gui-jin, YAO Xin-miao. Network Pharmacology-Based Study on the mechanism and targets of Yigu Decoction in the treatment of osteoporosis[J]. Chinese Journal of General Practice, 2021, 19(9): 1578-1583. doi: 10.16766/j.cnki.issn.1674-4152.002116
Citation: CHEN Zhi-neng, XIE Li-li, CHEN Hua, YAN Kun, LI Gui-jin, YAO Xin-miao. Network Pharmacology-Based Study on the mechanism and targets of Yigu Decoction in the treatment of osteoporosis[J]. Chinese Journal of General Practice, 2021, 19(9): 1578-1583. doi: 10.16766/j.cnki.issn.1674-4152.002116
shu

Network Pharmacology-Based Study on the mechanism and targets of Yigu Decoction in the treatment of osteoporosis

doi: 10.16766/j.cnki.issn.1674-4152.002116
  • 1.

    Department of Orthopedics and Traumatology, the Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 311005, China

Funds:

 LZ15H270001

 LY17H270005

 浙卫办中医[2019]1号

  • Received Date: 2020-03-10
    Available Online: 2022-02-15
    Abstract
  •   Objective  To investigate the molecular basis of Yigu Decoction (YGD) and its predictive targets and clarify the potential targets and mechanisms of YGD in the treatment of osteoporosis.  Methods  The active components and their targets corresponding to the herbs by traditional Chinese medicine systems pharmacology database (TCMSP) were determined via network pharmacology. The relevant targets of YGD in the treatment of osteoporosis were predicted using OMIM and GeneCards databases. A network of 'drug component-target disease' was constructed. A component-based target network for the treatment of osteoporosis with YGD components was constructed using Cytoscape software. A protein-protein interaction (PPI) network was constructed using STRING database. Gene Ontology (GO) enrichment and KEGG signalling pathways of the targets were analysed using a biological information annotation database.  Results  A total of 159 effective active components and 589 drug targets related to six herbs were found in YGD. Protein interaction analysis suggested that INS, AKT1, IL6, MAPK3 and VEGFA were the core targets of protein interaction network. A total of 334 targets were retrieved according to their orders, which were further enriched in 3 148 GO terms (all P < 0.05). A total of 175 signalling pathways were significantly enriched (all P < 0.05), including the PI3K-Akt signalling pathway, the MAPK signalling pathway, the cGMP-PKG signalling pathway, the Wnt signalling pathway and osteoclast differentiation.  Conclusion  The herbs, compounds and targets related to the compounds of YGD are effective carriers for YGD according to their orders. The targets and pathways of YGD not only regulate the functions of osteoblasts and osteoclasts but also intervene in bone microcirculation, which reflect the material basis of the treatment of osteoporosis by Bushen Jianpi Huoxue and conforms to the pathophysiological mechanism of osteoporosis.

     

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