Volume 20 Issue 1
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(1): 22-26, 53
WANG Yong-chao, ZHENG Zeng-guang. Effect and mechanism of berberine-targeted regulation of TLR4 signaling pathway on the biological behaviour of malignant melanoma[J]. Chinese Journal of General Practice, 2022, 20(1): 22-26, 53. doi: 10.16766/j.cnki.issn.1674-4152.002267
Citation: WANG Yong-chao, ZHENG Zeng-guang. Effect and mechanism of berberine-targeted regulation of TLR4 signaling pathway on the biological behaviour of malignant melanoma[J]. Chinese Journal of General Practice, 2022, 20(1): 22-26, 53. doi: 10.16766/j.cnki.issn.1674-4152.002267
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Effect and mechanism of berberine-targeted regulation of TLR4 signaling pathway on the biological behaviour of malignant melanoma

doi: 10.16766/j.cnki.issn.1674-4152.002267

  • Department of Pathology, The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, Zhejiang 310022, China

Funds:

 2018252439

  • Received Date: 2020-07-14
    Available Online: 2022-03-03
    Abstract
  •   Objective  To explore the regulatory effect of berberine on the TLR4 signaling pathway in malignant melanoma, to evaluate its effect on the proliferation, migration, and apoptosis of malignant melanoma A375 cells induced by lipopolysaccharide (LPS), and to provide a new drug (berberine) treatment basis for the prevention and treatment of malignant melanoma.  Methods  Western blotting was used to detect the intervention effects of different concentrations of berberine on the expression of TLR4 and NF-κB in A375 cells. The experimental groups were as follows: group A was control group, group B was LPS group, group C was TAK-242 group, group D was LPS+TAK-242 group, group E was LPS+TAK-242+80 μmol/L berberine group, and group F was LPS+TAK-242+120 μmol/L Berberine group. CCK-8, Transwell and flow cytometry assays were used to detect the effects of berberine targeting TLR4 on the proliferation, migration, and apoptosis of A375. We used enzyme-linked immunosorbent assay to detect the expression of IL-10 and TGF-β in the supernatant of each group of cell influences.  Results  With increased concentration of berberine, the expression of TLR4 and NF-κB protein was down-regulated more significantly (all P < 0.05). Compared with group A, the proliferation and migration ability of group B was enhanced, but no difference existed in apoptosis. The proliferation and migration ability of group C was weakened, and apoptosis was increased. The proliferation and migration ability of group D was weaker than that of group B and stronger than that of group C. Compared with group D, the proliferation and migration ability of E and F groups weakened, and apoptosis increased. At high concentrations of berberine, the cell proliferation and migration ability weakened more obviously (all P < 0.05). The expression levels of IL-10 and TGF-β in group B increased (all P < 0.05), the expression level of IL-10 in group C decreased, and the expression of TGF-β did not change significantly. The IL-10 of group D was lower than that of group B and increased compared with that of group C. The expression levels of IL-10 and TGF-β in group F were significantly reduced (all P < 0.05).  Conclusion  Berberine inhibits the proliferation and migration of LPS-induced malignant melanoma cells and promotes their apoptosis by inhibiting the TLR4/NF-κB signaling pathway. Berberine may be used as a medicine to treat malignant melanoma.

     

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