Volume 20 Issue 1
Jan.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(1): 43-46
HUANG Yi-bo, NIU Ying-qun, HUANG Guo-qiang, HU Ru-ying. Establishment and verification of a prognostic model for patients with gastric cancer based on clock gene promoter methylation[J]. Chinese Journal of General Practice, 2022, 20(1): 43-46. doi: 10.16766/j.cnki.issn.1674-4152.002272
Citation: HUANG Yi-bo, NIU Ying-qun, HUANG Guo-qiang, HU Ru-ying. Establishment and verification of a prognostic model for patients with gastric cancer based on clock gene promoter methylation[J]. Chinese Journal of General Practice, 2022, 20(1): 43-46. doi: 10.16766/j.cnki.issn.1674-4152.002272
shu

Establishment and verification of a prognostic model for patients with gastric cancer based on clock gene promoter methylation

doi: 10.16766/j.cnki.issn.1674-4152.002272
  • 1.

    Department of General Surgery, the Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310005, China

Funds:

 2017KY517

 2014KYB180

  • Received Date: 2020-07-09
    Available Online: 2022-03-03
    Abstract
  •   Objective  To establish and certificate a prognostic model for gastric cancer patients based on methylation of the promoter region of the clock gene.  Methods  A total of 459 patients with gastric cancer admitted to the Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine from January 2015 to January 2018 were selected. They were followed up for 2 years after operation and were divided into the recurrence group and non-recurrence group according to the recurrence. The differences in the general data, surgery-related data and methylation frequency of the clock gene promoter region between the two groups were compared. Multivariate logistic regression analysis was performed to determine the independent influencing factors of recurrence, and R software was used to establish a predictive model of recurrence and verify its efficacy.  Results  During follow-up, 5 cases were lost to follow-up. Among the remaining 454 patients, 217 had recurrence and were classified as the recurrence group, and the remaining 237 cases without recurrence were classified as the non-recurrence group. The recurrence rate of gastric cancer was 47.80% (217/454). There were statistically significant differences in the degree of differentiation, lymph node metastasis, tumour growth pattern and methylation frequency of the per1, per2, cry1 and clock gene promoter regions between the two groups (P < 0.05). Multivariate logistic regression analysis showed that the degree of differentiation (OR=0.519, P < 0.05), lymph node metastasis (OR=2.365, P < 0.05), tumour growth pattern (OR=0.616, P < 0.05), per1 (OR=24.108, P < 0.05), per2 (OR=2.152, P < 0.05), cry1 (OR=3.131, P < 0.05) and clock (OR=1.676, P < 0.05) were independent factors affecting gastric cancer recurrence. The prediction model showed that the consistency index of recurrence was 0.906 (95% CI: 0.879-0.938).  Conclusion  The degree of differentiation, lymph node metastasis, tumour growth pattern and methylation of the per1, per2, cry1 and clock gene promoter regions as influencing factors can effectively predict the recurrence of gastric cancer, and have good application value.

     

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