Volume 20 Issue 1
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(1): 152-156
MAO Guo-feng, ZHOU Jie, LYU Li. Analysis of virulence and drug resistance of pan drug resistant Klebsiella pneumoniae[J]. Chinese Journal of General Practice, 2022, 20(1): 152-156. doi: 10.16766/j.cnki.issn.1674-4152.002300
Citation: MAO Guo-feng, ZHOU Jie, LYU Li. Analysis of virulence and drug resistance of pan drug resistant Klebsiella pneumoniae[J]. Chinese Journal of General Practice, 2022, 20(1): 152-156. doi: 10.16766/j.cnki.issn.1674-4152.002300
shu

Analysis of virulence and drug resistance of pan drug resistant Klebsiella pneumoniae

doi: 10.16766/j.cnki.issn.1674-4152.002300
  • 1.

    Department of Clinical Laboratory, Shaoxing People's Hospital, Shaoxing, Zhejiang 312000, China

Funds:

 LGF20H200004

  • Received Date: 2020-05-04
    Available Online: 2022-03-03
    Abstract
  •   Objective  To identify resistance genes, virulence factors and homology of two strains of pan drug resistant Klebsiella pneumoniae with different origins.  Methods  Two strains of pan drug resistant bacteria were isolated from the urine of two inpatients in early 2019. The minimal inhibitory concentration of the strain was determined by broth dilution, and the carbapenemase phenotype was screened by EDTA and PBA. Using the third-generation sequencing platform for genome sequencing and screening of drug-resistant genes and virulence factors, the sequencing Results were spliced, assembled and annotated. Multilocus sequence typing (MLST) was conducted for genotyping of strains.  Results  The two strains were resistant to most antibiotics including carbapenems except tigecycline and polymyxin. The synergistic test of EDTA and PBA suggested that carbapenemase might be carried. The two strains all carried one chromosome and two plasmids. The difference of chromosome genes was 1 192 bp, and the number of plasmids was the same. β-lactam resistance genes such as SHV-28, DHA-18, NMC, SPM and AIM, efflux system such as AcrA-AcrB-TolC, and outer membrane protein deletions of OmpK35 and OmpK36 were detected. The virulence factors included iron uptake system, pili, capsule, endotoxin and binary regulatory system. All MLST types were ST15.  Conclusion  β-lactam resistance genes combined with the loss of outer membrane proteins are the main reasons for the resistance of bacteria to carbapenems. NMC, SPM and AIM β-lactam resistance genes are detected in Klebsiella pneumoniae for the first time. There may be clonal transmission of pan drug resistant Klebsiella pneumoniae.

     

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