Level of autophagy-related factors in colorectal adenocarcinoma and its relationship with clinical pathological characteristics

XUE Jian-feng; WEI Na; WANG Xi-jiao; YANG Chun

doi:10.16766/j.cnki.issn.1674-4152.002353

Volume 20 Issue 3

Mar. 2022

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Article Navigation > Chinese Journal of General Practice > 2022 > 20(3): 362-365

XUE Jian-feng, WEI Na, WANG Xi-jiao, YANG Chun. Level of autophagy-related factors in colorectal adenocarcinoma and its relationship with clinical pathological characteristics[J]. Chinese Journal of General Practice, 2022, 20(3): 362-365. doi: 10.16766/j.cnki.issn.1674-4152.002353

Citation:

XUE Jian-feng, WEI Na, WANG Xi-jiao, YANG Chun. Level of autophagy-related factors in colorectal adenocarcinoma and its relationship with clinical pathological characteristics[J]. Chinese Journal of General Practice, 2022, 20(3): 362-365. doi: 10.16766/j.cnki.issn.1674-4152.002353

Citation:

XUE Jian-feng, WEI Na, WANG Xi-jiao, YANG Chun. Level of autophagy-related factors in colorectal adenocarcinoma and its relationship with clinical pathological characteristics[J]. Chinese Journal of General Practice, 2022, 20(3): 362-365. doi: 10.16766/j.cnki.issn.1674-4152.002353

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Level of autophagy-related factors in colorectal adenocarcinoma and its relationship with clinical pathological characteristics

doi: 10.16766/j.cnki.issn.1674-4152.002353

XUE Jian-feng¹
, WEI Na^{1, 2
,
,}
, WANG Xi-jiao²
, YANG Chun¹

1.
Department of Pathology, Guizhou Medical University, Guiyang, Guizhou 550000, China

Funds:

U1812403-6

81560512

Received Date: 2021-10-20
Available Online: 2022-08-13

Abstract

Abstract

Objective To investigate the expression of autophagy-related factors Unc-51 like autophagy activating kinase 1(ULK1), B cell lymphoma-2-interacting protein(Beclin1) and autophagy marker light chain 3(LC3) in colorectal adenocarcinoma and their correlation with clinicopathological features. Methods A total of 63 cases with complete data of radical resection of colorectal cancer from January 2020 to December 2020 in the Department of Pathology of the Affiliated Hospital of Guizhou Medical University were collected. Immunohistochemical EnVision method was used to detect the expression of autophagy-related factors ULK1, Beclin1 and LC3 in tumour tissues and corresponding precancerous tissues. The correlation between autophagy-related protein expression and clinicopathological data such as age, sex, primary location, nerve invasion, differentiation and lymph node metastasis were analysed. Results Autophagy related proteins ULK1, Beclin1 and LC3 were mainly expressed in cytoplasm. Compared with normal tissues, the protein expression levels of ulk1 (71.43% vs. 44.44%), Beclin1 (66.66% vs. 47.61%) and LC3 (55.55% vs. 30.15%) in intestinal adenocarcinoma were significantly higher (all P < 0.05). ULK1 was correlated with tumor differentiation (P < 0.05), while Beclin1 and LC3 showed no significant difference between tumor tissue and normal tissue (all P>0.05). There was no significant correlation with age, sex, primary site, gross type and nerve invasion. In addition, ULK1 and LC3 were correlated with lymph node metastasis (all P < 0.05), while Beclin1 was not significantly correlated with lymph node metastasis (P>0.05). Conclusion The expression level of autophagy-related protein is increased in colorectal cancer, which is related to the degree of tissue differentiation. The expression level of some proteins was related to lymph node metastasis. The increased expression of autophagy in colorectal cancer may be one of the mechanisms of carcinogenesis and metastasis. Interventions targeting the autophagy pathway in tumour tissues may solve some problems of recurrence and metastasis of colorectal cancer.
- Autophagy,
- Colorectal cancer,
- B cell lymphoma-2-interacting protein,
- Unc-51 like autophagy activating kinase 1,
- Autophagy marker light chain 3

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References

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Level of autophagy-related factors in colorectal adenocarcinoma and its relationship with clinical pathological characteristics

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