Volume 20 Issue 5
May  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(5): 781-784
GU Shao-jun, QIAN Chun-yan, FU Huan-quan. Experimental study of miR-613 targeting PTBP1 to inhibit the invasion and migration of endometrial cancer cells[J]. Chinese Journal of General Practice, 2022, 20(5): 781-784. doi: 10.16766/j.cnki.issn.1674-4152.002453
Citation: GU Shao-jun, QIAN Chun-yan, FU Huan-quan. Experimental study of miR-613 targeting PTBP1 to inhibit the invasion and migration of endometrial cancer cells[J]. Chinese Journal of General Practice, 2022, 20(5): 781-784. doi: 10.16766/j.cnki.issn.1674-4152.002453
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Experimental study of miR-613 targeting PTBP1 to inhibit the invasion and migration of endometrial cancer cells

doi: 10.16766/j.cnki.issn.1674-4152.002453
  • 1.

    Department of Laboratory, the First People's Hospital of Xiaoshan District, Hangzhou, Zhejiang 311200, China

Funds:

 LGF19H200002

  • Received Date: 2021-11-21
    Available Online: 2022-09-05
    Abstract
  •   Objective  To investigate the effects of miR-613 targeting polypyrimidine tract-binding protein 1 (PTBP1) on the proliferation, migration and invasion of endometrial cancer cells (Ishikawa).  Methods  Real-time fluorescent quantitative PCR (RT-qPCR) was conducted to detect the expression of miR-613 and PTBP1 mRNA in endometrial cancer tissues. Ishikawa cells were cultured in vitro, and intervened to overexpress miR-613, inhibit PTBP1 expression, or co-overexpress miR-613 and PTBP1. The proliferation, migration and invasion of Ishikawa cells and the protein expression levels of PTBP1, matrix metalloproteinase-2(MMP-2) and MMP-9 were detected respectively. Dual-luciferase reporter assay was conducted to verify the targeting relationship between miR-613 and PTBP1.  Results  The expression level of miR-613 in endometrial cancer tissue was 0.48±0.09, which was significantly lower than that in paracancerous tissue (1.03±0.11, P < 0.05). The expression level of PTBP1 mRNA was 2.78±0.23, which was significantly higher than that in paracancerous tissue (1.01±0.12, P < 0.05). MiR-613 was negatively correlated with PTBP1 mRNA (r=-0.523, P < 0.05). The results of dual-luciferase reporter gene assay showed that miR-613 targeted and negatively regulated the expression of PTBP1. Overexpression of miR-613 and inhibition of PTBP1 expression significantly decreased the survival rate of Ishikawa cells, the number of cell migration and invasion, and the expression levels of PTBP1, MMP-2 and MMP-9 (all P < 0.05). Overexpression of PTBP1 reversed the inhibitory effects of overexpression of miR-613 on Ishikawa cell proliferation, migration and invasion.  Conclusion  The expression of miR-613 is low in endometrial cancer tissues. Overexpression of miR-613 can inhibit the proliferation, migration and invasion of human endometrial cancer cells by targeting and inhibiting the expression of PTBP1.

     

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