Volume 20 Issue 5
May  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(5): 828-831
WANG Mei, ZHANG Lin, CUI Fa-cai, WU Xiao-yu. The expression level of serum miR-96 and miR-424-5p in patients with colorectal cancer and its clinical significance[J]. Chinese Journal of General Practice, 2022, 20(5): 828-831. doi: 10.16766/j.cnki.issn.1674-4152.002465
Citation: WANG Mei, ZHANG Lin, CUI Fa-cai, WU Xiao-yu. The expression level of serum miR-96 and miR-424-5p in patients with colorectal cancer and its clinical significance[J]. Chinese Journal of General Practice, 2022, 20(5): 828-831. doi: 10.16766/j.cnki.issn.1674-4152.002465
shu

The expression level of serum miR-96 and miR-424-5p in patients with colorectal cancer and its clinical significance

doi: 10.16766/j.cnki.issn.1674-4152.002465
  • 1.

    Department of Clinical Laboratory, Henan Provincial Veteran's Hospital, Xinxiang, Henan 453003, China

Funds:

 201702220

  • Received Date: 2021-09-11
    Available Online: 2022-09-05
    Abstract
  •   Objective  To investigate the relationship between the expression of serum miR-96 and miR-424-5p and the clinicopathological characteristics and prognosis of patients with colorectal cancer (CRC), as well as explore the diagnostic value of serum miR-96 and miR-424-5p detection in CRC.  Methods  The serum samples of 120 patients with CRC, 40 patients with colorectal adenomas (CRAs) and 40 healthy controls from January to December 2015 in Henan Provincial People's Hospital were collected. The expression levels of miR-96 and miR-424-5p were detected by real-time quantitative PCR (RT-PCR). The relationship between the expression of miR-96 and miR-424-5p and the clinicopathological characteristics or the prognosis of patients with CRC was analysed. The receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic efficacy of serum miR-96 and miR-424-5p.  Results  The expression levels of serum miR-96 and miR-424-5p in patients with CRC were significantly higher than those in patients with CRA or healthy controls, with statistically significant differences (all P < 0.05). The expression level of serum miR-96 in patients with TNM stage Ⅲ/Ⅳ, low differentiation and distant metastasis was higher than those in patients with TNM Ⅰ/Ⅱ, moderate or high differentiation and without distant metastasis (all P < 0.01). The expression level of serum miR-424-5p in patients with TNM stage Ⅲ/Ⅳ was higher than that in patients with TNM Ⅰ/Ⅱ (all P < 0.01). The 5-year survival rate and mean survival time of patients with high expression of serum miR-96 and miR-424-5p were lower than those with low expression of serum miR-96 and miR-424-5p (all P < 0.01). The area under the curve (AUC) and specificity of miR-96 versus miR-424-5p in CRC were 0.727 and 71.2% versus 0.720 and 86.2%, respectively. The combined detection of AUC or specificity was 0.780 and 93.7%, which was greater than any single detection (all P < 0.05).  Conclusion  The increased expression levels of serum miR-96 and miR-424-5p were correlated with the occurrence, development and poor prognosis of CRC. The combined detection of serum miR-96 and miR-424-5p has significant reference value for the diagnosis of CRC.

     

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