Effects of tobacco exposure during pregnancy on pregnant women and foetuses based on placental gene expression profiling

LIU Xuan-shi; HAO Chan-juan; XU Li-xin; LI Wei

doi:10.16766/j.cnki.issn.1674-4152.002593

Volume 20 Issue 8

Aug. 2022

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Article Navigation > Chinese Journal of General Practice > 2022 > 20(8): 1346-1349

YU Li-ming, SHI Xin-yan, CHEN Yi-ming, SHEN Yong-hai, WANG Qiu-meng, HU Wen-sheng. Comparison of seven bile acids in intrahepatic cholestasis by HPLC-MS/MS at different gestational stages and the clinical significance[J]. Chinese Journal of General Practice, 2020, 18(7): 1153-1156,1157. doi: 10.16766/j.cnki.issn.1674-4152.001453

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LIU Xuan-shi, HAO Chan-juan, XU Li-xin, LI Wei. Effects of tobacco exposure during pregnancy on pregnant women and foetuses based on placental gene expression profiling[J]. Chinese Journal of General Practice, 2022, 20(8): 1346-1349. doi: 10.16766/j.cnki.issn.1674-4152.002593

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Effects of tobacco exposure during pregnancy on pregnant women and foetuses based on placental gene expression profiling

doi: 10.16766/j.cnki.issn.1674-4152.002593

1.
Beijing Key Laboratory for Genetics of Birth Defects, Beijing Paediatric Research Institute; MOE Key Laboratory of Major Diseases in Children; Genetics and Birth Defects Control Centre, Beijing Children' s Hospital, Capital Medical University, National Centre for Children' s Health, Beijing 100045, China

Funds:

2016YFC1000306

5222007

Received Date: 2021-12-15

Abstract

Abstract

Objective To expand the understanding of the molecular mechanisms underlying tobacco exposure during pregnancy on pregnant women and their foetuses. Methods Firstly, the NCBI GEO database and EBI ArrayExpress database were systematically searched, and two gene expression profile datasets from placental tissues of smoking and non-smoking pregnant women were selected. After integrating and correcting these datasets, various bioinformatics methods and models including differential expression, functional annotation, enrichment analysis and regression analysis were applied to study the effects of tobacco exposure on pregnant women and foetuses. Results Amongst 476 significantly differentially expressed genes (FDR < 0.5, |log₂FC|>0.3), 317 were up-regulated and 159 were down-regulated. After functional annotations and enrichment analyses using KEGG, GO, Reactome and MSigDb, these genes mainly appeared in extracellular structure (P=1.10×10^-28), extracellular matrix (P=3.44×10^-25) and mesenchymal development (P=2.60×10^-13). Regression analysis was conducted to study molecular markers of tobacco exposure. CDCA7L was the most significant gene correlated with cotinine level after adjusting for confounding factors (FDR=0.046). Conclusion The pathways and the possible molecular mechanisms that pregnant women and foetuses may be affected by tobacco exposure are explored and discussed, and the ideas for future molecular mechanism research are proposed.
- Pregnancy,
- Gene expression profiles,
- Tobacco exposure,
- Placenta,
- Differential expression,
- Enrichment analysis,
- Regression analysis

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References

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Effects of tobacco exposure during pregnancy on pregnant women and foetuses based on placental gene expression profiling

doi: 10.16766/j.cnki.issn.1674-4152.002593