Volume 20 Issue 9
Sep.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(9): 1548-1550
ZHOU Ya-di, JIN Fa-xiang. Identification of nontuberculous mycobacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and analysis of drug-sensitivity test[J]. Chinese Journal of General Practice, 2022, 20(9): 1548-1550. doi: 10.16766/j.cnki.issn.1674-4152.002646
Citation: ZHOU Ya-di, JIN Fa-xiang. Identification of nontuberculous mycobacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and analysis of drug-sensitivity test[J]. Chinese Journal of General Practice, 2022, 20(9): 1548-1550. doi: 10.16766/j.cnki.issn.1674-4152.002646
shu

Identification of nontuberculous mycobacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and analysis of drug-sensitivity test

doi: 10.16766/j.cnki.issn.1674-4152.002646

  • Department of Clinical Laboratory, Affiliated Hospital of Shaoxing University (Shaoxing Municipal Hospital), Shaoxing, Zhejiang 312000, China

Funds:

 2020KY334

  • Received Date: 2022-03-04
    Available Online: 2022-11-29
    Abstract
  •   Objective  To study the typing results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) of nontuberculous mycobacteria (NTM), compare the drug resistance of different NTM strains, and provide a basis for clinical rational drug use.  Methods  The data on NTM strains in Shaoxing City Tuberculosis Designated Hospital (Affiliated Hospital of Shaoxing College of Arts and Sciences) from March 2019 to June 2021 were retrospectively analyzed. After excluding the strain data from the same patient, the remaining 128 NTM strains were classified by MALDI-TOF MS technology. Genotyping was performed at the same time, and the results of genotyping were used as the gold standard. The accuracy of MALDI-TOF MS typing results was calculated, and drug susceptibility tests were performed on the top three NTMs after typing.  Results  The accuracy of NTM typing by MALDI-TOF MS was 98.4%. The sensitivity rates of mycobacterium intracellular to amikacin (AK), clarithromycin (CLA), linezolid (LZD), moxifloxacin (MXF) and rifampicin (RFP) were 98.5%, 89.4%, 86.4%, 83.3% and 62.1%, respectively. The sensitivity rates of mycobacterium avium to AK, CLA, LZD, MXF and RFP were 100.0%, 92.9%, 100.0%, 82.1% and 89.3%, respectively. The drug resistance rates of the above strains to minocycline (MH) were 90.9% and 82.1%, respectively. The sensitivity rates of mycobacterium Kansas to AK, CLA and RFP were 100.0%, 100.0% and 95.2%, respectively.  Conclusion  MALDI-TOF MS has high accuracy in NTM typing, and mycobacterium intracellular, Mycobacterium avium and Mycobacterium Kansas are highly sensitive to AK and CLA. AK and CLA can be used as the first choice for clinical treatment of NTM.

     

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