Volume 20 Issue 10
Oct.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(10): 1654-1657
XU Feng, XU Lin-xia, LIU Wei, WANG Ke-ke, CHEN Cai-feng, Xie Li, KE Xi-quan. The molecular mechanism of sinomenine hydrochloride treat ulcerative colitis through Hedgehog signalling pathway in mice[J]. Chinese Journal of General Practice, 2022, 20(10): 1654-1657. doi: 10.16766/j.cnki.issn.1674-4152.002671
Citation: XU Feng, XU Lin-xia, LIU Wei, WANG Ke-ke, CHEN Cai-feng, Xie Li, KE Xi-quan. The molecular mechanism of sinomenine hydrochloride treat ulcerative colitis through Hedgehog signalling pathway in mice[J]. Chinese Journal of General Practice, 2022, 20(10): 1654-1657. doi: 10.16766/j.cnki.issn.1674-4152.002671
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The molecular mechanism of sinomenine hydrochloride treat ulcerative colitis through Hedgehog signalling pathway in mice

doi: 10.16766/j.cnki.issn.1674-4152.002671

  • Department of Gastroenterology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 gxgwfx2018052

  • Received Date: 2022-03-30
    Available Online: 2022-11-30
    Abstract
  •   Objective  To investigate the protective effect and possible mechanism of sinomenine hydrochloride (SH) on ulcerative colitis (UC) in mice.   Methods  Twenty-four mice were randomly divided into 4 groups: normal group, model group, low and high dose SH group (SH 20 mg/kg, 60 mg/kg), with 6 mice in each group. Disease activity index (DAI) and colon histopathology (HI) scores were recorded, and the expression levels of Shh mRNA and related proteins and cytokines were detected.   Results  Compared with the normal group, the DAI score [(3.44±0.27) points vs. 0, P < 0.001] and HI score [(7.67±0.52) points vs. 0, P < 0.001] increased, Shh mRNA (P < 0.05), Shh, Smo, Ptch1 and Gli1 protein (all P < 0.01) decreased, and TNF-α, IL-1β and IL-6 (all P < 0.001) increased in the model group. Compared with the model group, the DAI score [(2.50±0.18) points, (1.89±0.17) points vs. (3.44±0.27) points, P < 0.001], HI score [(5.17±0.75) points, (3.33±0.52) points vs. (7.67±0.52) points, P < 0.001] decreased, Shh, Smo, Ptch1 and Gli1 protein (all P < 0.001) increased, and TNF-α, IL-1β, IL-6 (all P < 0.01) decreased in the SH treatment group.   Conclusion  SH can treat ulcerative colitis in mice. The mechanism may be that it up regulates the activity of Hedgehog signalling pathway, thereby reduces the content of TNF-α, IL-1β, IL-6.

     

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