Volume 20 Issue 12
Dec.  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(12): 2029-2033
JIN Yue-wei, MAO Zu-jie, HU Lin-yi, PAN Yong-zhi, WANG Jun-jie, YANG Pan, ZHENG Min-chao, ZHENG Yong-qiang, ZHU Long. Mechanism of miR-373-3p in reversing cisplatin resistance in bladder cancer cells by regulating caveolin 1[J]. Chinese Journal of General Practice, 2022, 20(12): 2029-2033. doi: 10.16766/j.cnki.issn.1674-4152.002762
Citation: JIN Yue-wei, MAO Zu-jie, HU Lin-yi, PAN Yong-zhi, WANG Jun-jie, YANG Pan, ZHENG Min-chao, ZHENG Yong-qiang, ZHU Long. Mechanism of miR-373-3p in reversing cisplatin resistance in bladder cancer cells by regulating caveolin 1[J]. Chinese Journal of General Practice, 2022, 20(12): 2029-2033. doi: 10.16766/j.cnki.issn.1674-4152.002762
shu

Mechanism of miR-373-3p in reversing cisplatin resistance in bladder cancer cells by regulating caveolin 1

doi: 10.16766/j.cnki.issn.1674-4152.002762
  • 1.

    Department of Urology Surgery, East and South Zhejiang Hospital of Zhejiang Provincial

Funds:

 2019RC102

 20ywb178

  • Received Date: 2022-11-02
    Available Online: 2023-02-07
    Abstract
  •   Objective  To investigate the effect of microRNA-373-3p (miR-373-3p) targeting caveolin 1 (Cav1) on the cisplatin (DDP) resistance of bladder cancer cell.  Methods  The DDP-resistant bladder cancer cell line T24/DDP were established. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-373-3p in T24 and T24/DDP cells. miR-373-3p was overexpressed, or the expression of Cav1 was inhibited in T24/DDP cells. Each group of experiments was set up with six replicate wells. MTT method was used to detect the half inhibitory concentration (IC50) of DDP on T24/DDP cells and cell proliferation activity. Flow cytometry was used to detect cell apoptosis, and Western blotting was used to determine the expressions of Cav1, multidrug resistance associated protein (MRP) and P-glycoprotein (P-gp) proteins in cells. Dual luciferase reporter gene experiment was used to verify the targeting relationship between miR-373-3p and Cav1.  Results  Compared with T24 cells, the expression level of miR-373-3p in T24/DDP cells decreased (0.34±0.06 vs. 1.00±0.18, t=8.521, P < 0.001), and that of Cav1 protein increased (0.95±0.10 vs. 0.41±0.06, t=11.342, P < 0.001). Dual luciferase reporter experiment proved that miR-373-3p could target and negatively regulate Cav1 expression. After the overexpression of miR-373-3p or inhibition of Cav1 expression in T24/DDP cells, the apoptosis rate significantly increased (all P < 0.05), and the cell proliferation activity, IC50 of DDP to the cell and protein expression levels of MRP and P-gp significantly decreased (all P < 0.05). The overexpressed Cav1 could reverse the inhibitory effect of the overexpression of miR-373-3p on the drug resistance of T24/DDP cells.  Conclusion  MiR-373-3p can target and down regulate Cav1 expression and reverse the DDP resistance of T24/DDP cells.

     

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