Volume 21 Issue 1
Jan.  2023
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(1): 41-44
CHENG Feng, QIU Zhao-lei, ZHENG Chuan-ming, XIA Ya-qin, LI Lei, DU Zhao-hui, JIANG Hai, WANG Zhen-jie, XIE Fu-chen. Mechanism of JAK/STAT signaling pathway in the early stage severe acute pancreatitis rats[J]. Chinese Journal of General Practice, 2023, 21(1): 41-44. doi: 10.16766/j.cnki.issn.1674-4152.002807
Citation: CHENG Feng, QIU Zhao-lei, ZHENG Chuan-ming, XIA Ya-qin, LI Lei, DU Zhao-hui, JIANG Hai, WANG Zhen-jie, XIE Fu-chen. Mechanism of JAK/STAT signaling pathway in the early stage severe acute pancreatitis rats[J]. Chinese Journal of General Practice, 2023, 21(1): 41-44. doi: 10.16766/j.cnki.issn.1674-4152.002807
shu

Mechanism of JAK/STAT signaling pathway in the early stage severe acute pancreatitis rats

doi: 10.16766/j.cnki.issn.1674-4152.002807
  • 1.

    Department of Emergency Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 KJ2019A0385

 BYKY1779

 S201910367121

 BYKY2019095ZD

 2021byzd160

  • Received Date: 2022-01-22
    Available Online: 2023-04-07
    Abstract
  •   Objective  To investigate the effect of inhibiting JAK/STAT signaling pathway on relevant disease indicators in rats with early stage severe acute pancreatitis (SAP), so as to speculate the possible mechanism of this signaling pathway in SAP rats.  Methods  Fifty-four adult SD rats were divided into control group, SAP group and AG490 group according to random number table, 18 rats in each group. AG490 group was given AG490 application before modeling, other groups were given equal amount of saline. SAP model was made by retrograde intra-biliary pancreatic duct injection with 5% sodium taurocholate, and the rats were executed at 6 h, 12 h and 24 h after modeling, and atrial blood was taken to measure serum amylase, calcium, TNF-α and calcium. The pancreatic tissues were examined by HE staining. Western blotting was used to detect the expression level of STAT3 protein in pancreatic tissues.  Results  The amylase values in the AG490 group at 6 h, 12 h and 24 h were (2 049.17±257.00) U/L, (2 915.00±188.42) U/L and (3 746.50±181.05) U/L, respectively, which were higher than those in the control group and lower than those in the SAP group, and SAP group was higher than the control group (all P < 0.05). The blood calcium concentrations at 6 h, 12 h and 24 h in the AG490 group were higher than those in the SAP group, both groups were lower than the control group (all P < 0.05). The TNF-α, IL-6 and IL-1 at 6 h, 12 h and 24 h in the AG490 group were lower than those in the SAP group, and both groups were higher than the control group. STAT3 expression in SAPgrouppancreatic tissue increased with time between groups, and the 24 h point was significantly higher than the AG490 group, with insignificant differences at other points. AG490 group, pancreatic cell edema and inflammatory cell infiltration were seen in pathological sections, no significant difference.  Conclusion  The early stage of severe acute pancreatitis may induce multiple inflammatory factors through the JAK/STAT signaling pathway and promote the progression of pancreatitis.

     

    • FullText(HTML)
  • loading
    • References(17)
  • [1]
    FEI S K, LI W, XIANG L, XIE X W, et al. Protective effect of alprostadil on acute pancreatitis in rats via inhibiting Janus kinase 2 (JAK2)/STAT3 signal transduction pathway[J]. Med Sci Monit, 2019, 25: 7694-7701. DOI: 10.12659/MSM.919148.
    [2]
    中华医学会外科学分会胰腺外科学组. 中国急性胰腺炎诊治指南(2021)[J]. 中华外科杂志, 2021, 59(7): 578-587. doi: 10.3760/cma.j.cn112139-20210416-00172

    Pancreatic Surgery Group, Surgery Society of Chinese Medical Association. Chinese Guidelines for Diagnosis and treatment of acute pancreatitis (2021)[J]. Chinese Journal of Surgery, 2021, 59(7): 578-587. doi: 10.3760/cma.j.cn112139-20210416-00172
    [3]
    MONTERO P, MILARA J, ROGER I, et al. Role of JAK/STAT in interstitial lung diseases; molecular and cellular mechanisms[J]. Int J Mol Sci, 2021, 22: 6211. DOI: 10.3390/ijms22126211.
    [4]
    CAO W Y, YANG D L, HOU S L. PCT、IL-6、hs-CRP对急性胰腺炎的诊断价值[J]. 检验医学与临床, 2019, 16(23): 3441-3443. doi: 10.3969/j.issn.1672-9455.2019.23.014
    [5]
    QIN L Y, WANG G X. 乌司他丁联合生长抑素对重症急性胰腺炎大鼠血清炎性细胞因子的影响[J]. 临床和实验医学杂志, 2015, 14(15): 1225-1228. doi: 10.3969/j.issn.1671-4695.2015.015.001
    [6]
    SU Z H, ZHENG L F, YANG H C. 早期血液滤过治疗急性重症胰腺炎合并间隔室综合征的疗效及对其血清IL-6、IL-8水平变化的影响[J]. 中国现代药物应用, 2017, 11(20): 21-23. https://www.cnki.com.cn/Article/CJFDTOTAL-ZWYY201720009.htm
    [7]
    WANG J F, LI N, LUO Q. 腹腔镜手术治疗重症急性胰腺炎及对TNF-α、IL-6和sIL-2R水平的影响[J]. 中国内镜杂志, 2017, 23(11): 74-78. doi: 10.3969/j.issn.1007-1989.2017.11.015
    [8]
    齐宏, 陈广, 王渊文, 等. 异甘草酸镁联合奥曲肽治疗急性胰腺炎的临床疗效及对hs-CRP、IL-1β、IL-6水平的影响[J]. 长春中医药大学学报, 2020, 36(3): 520-522. https://www.cnki.com.cn/Article/CJFDTOTAL-CZXX202003033.htm

    QI H, CHEN G, WANG Y W, et al. Effects of magnesium isoglycyrrhizinate combined with octreotide on acute pancreatitis and its effect on hs-CRP、IL-1β、IL-6 levels[J]. Journal of Changchun University of Chinese Medicine, 2020, 36(3): 520-522. https://www.cnki.com.cn/Article/CJFDTOTAL-CZXX202003033.htm
    [9]
    刘胜新, 徐军辉, 蔡涛, 等. IL-1β在急性胰腺炎时肝损伤中的作用[J]. 临床外科杂志, 2019, 27(9): 777-778. doi: 10.3969/j.issn.1005-6483.2019.09.017

    LIU S X, XU J H, CAI T, et al. The role of IL-1β in the acute pancreatitis with hepatic function damage[J]. Journal of Clinical Surgery, 2019, 27(9): 777-778. doi: 10.3969/j.issn.1005-6483.2019.09.017
    [10]
    沈银峰, 巴元明, 金文银, 等. JAK2-STAT3信号通路在急性坏死性胰腺炎大鼠胰腺损伤与全身炎症反应中的作用[J]. 中华胰腺病杂志, 2019, 19(3): 185-189. doi: 10.3760/cma.j.issn.1674-1935.2019.03.007

    SHEN Y F, BA Y M, JIN W Y, et al. Role of JAK2-STAT3 signaling pathway in pancreatic injury and systematic inflammatory response in rats with acute necrotizing pancreatitis[J]. Chinese Journal of Pancreatology, 2019, 19(3): 185-189. doi: 10.3760/cma.j.issn.1674-1935.2019.03.007
    [11]
    陈平, 姚玮艳, 章永平, 等. 雨蛙素诱导的急性胰腺炎体外细胞模型的信号转导通路研究[J]. 中华胰腺病杂志, 2018, 10(4): 272-275.

    CHEN P, YAO W Y, ZANG Y P, et al. Expression of janus kinase 1/signal transducer and activator of transcription 1 signaling pathway in cerulean stimulated pancreatic acinar cells[J]. Chinese J Ournal of Pancreatology, 2018, 10(4): 272-275.
    [12]
    WANG G L, ZHANG J C, DUI D H, et al. High mobility group box 1 induces the activation of the Janus kinase 2 and signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in pancreatic acinar cells in rats, while AG490 and rapamycin inhibit their activation[J]. Bosn J Basic Med Sci, 2016, 16(4): 307-312.
    [13]
    QIN M Z, QIN M B, LIANG Z H, et al. Effect of SOCS3 on lung injury in rats with severe acute pancreatitis through regulating JAK2/STAT3 signaling pathway[J]. Eur Rev Med Pharmacol Sci, 2019, 23(22): 10123-10131.
    [14]
    YANG X, GENG H, YOU L, et al. Rhein protects against severe acute pancreatitis in vitro and in vivo by regulating the JAK2/STAT3 pathway[J]. Front Pharmacol, 2022, 13: 778221. DOI: 10.3389/fphar.2022.778221.
    [15]
    ZHU S J, ZHANG C, WENG Q, et al. Curcumin protects against acute renal by suppressing JAK2/STAT3 pathway in severe acute pancreatitis in rats[J]. Exp Ther Med, 2017, 14(2): 1669-1674.
    [16]
    LI M L, ZHANG X H, WANG B, et al. Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats[J]. Exp Ther Med, 2018, 16(3): 2013-2021.
    [17]
    张剑, 魏殿军. 血淀粉酶、胰脂肪酶、PCT、CRP联合检测在急性胰腺炎中的诊断价值[J]. 中国实验诊断学, 2017, 21(5): 778-780. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSZD201705005.htm

    ZHANG J, WEI D J. Clinical significance of the combined detection of AMY, LPS, PCT and CRP in prognosis of acute pancreatitis[J]. Chinese Journal of Laboratory Diagnosis, 2017, 21(5): 778-780. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSZD201705005.htm
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)  / Tables(2)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (234) PDF downloads(5) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return