Volume 21 Issue 10
Oct.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(10): 1661-1664
ZHAO Pingge, ZHANG Yanan, ZHANG Wenya, XU Peng, HUANG Min, LI Meng, TENG Jiaqian, KUANG Ye. Role and mechanism of miR-429 in the progression of epithelial ovarian cancer[J]. Chinese Journal of General Practice, 2023, 21(10): 1661-1664. doi: 10.16766/j.cnki.issn.1674-4152.003194
Citation: ZHAO Pingge, ZHANG Yanan, ZHANG Wenya, XU Peng, HUANG Min, LI Meng, TENG Jiaqian, KUANG Ye. Role and mechanism of miR-429 in the progression of epithelial ovarian cancer[J]. Chinese Journal of General Practice, 2023, 21(10): 1661-1664. doi: 10.16766/j.cnki.issn.1674-4152.003194
shu

Role and mechanism of miR-429 in the progression of epithelial ovarian cancer

doi: 10.16766/j.cnki.issn.1674-4152.003194
  • 1.

    Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China

Funds:

 LH2021H034

  • Received Date: 2022-09-06
    Available Online: 2023-11-23
    Abstract
  •   Objective  To investigate the expression of miR-429 in epithelial ovarian cancer tissues and its relationship with the proliferation and migration of ovarian cancer cells, and to explore the role of miR-429 target gene DNAJB6 in ovarian cancer.  Methods  Twenty-seven epithelial ovarian cancer tissues and fourteen normal ovarian tissues obtained by gynecological surgical resection at the Second Affiliated Hospital of Harbin Medical University during the period from September 2019 to February 2022 were selected to detect the expression of miR-429 and DNAJB6 mRNA in the tissues using real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). In human ovarian cancer cell line A2780, miR-429 was knocked down and changes in tumor cell proliferation and migration ability were measured in vitro by using CCK-8 kit, plate clone formation and Transwell cell migration assay. The downstream target genes of miR-429 were predicted by bioinformatics software and its prognostic value in ovarian cancer was analyzed. RT-qPCR and Western blot assays were applied to further confirm the regulatory relationship between miR-429 and DNAJB6.  Results  Compared with normal ovarian tissues 0.886(0.785, 2.014), miR-429 was highly expressed in epithelial ovarian cancer tissues 2.048(0.830, 5.281), P=0.018, and overexpression of miR-429 could enhance the proliferation and migration of ovarian cancer cells. Compared with normal ovarian tissues (1.290±0.781), DNAJB6 was lowly expressed in epithelial ovarian cancer tissues (0.710±0.456, P=0.004), which was closely related to the prognosis of ovarian cancer patients. Knockdown of miR-429 upregulated DNAJB6 mRNA expression (2.839±0.305, P=0.001) compared to the control (1.020±0.251); knockdown of miR-429 also upregulated DNAJB6 protein expression (P=0.049).  Conclusion  miR-429 plays a pro-cancer role in epithelial ovarian cancer and can regulate the expression of the target gene DNAJB6, suggesting that miR-429/DNAJB6 may be a new target for ovarian cancer therapy.

     

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