Volume 21 Issue 10
Oct.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(10): 1702-1705
ZHANG Zhen, ZHUO Feixiang, WU Yumeng, CHEN Xin. Expression and clinical significance of miR-374a-5p and RAB10 in cord blood of neonates with hypoxic-ischemic encephalopathy[J]. Chinese Journal of General Practice, 2023, 21(10): 1702-1705. doi: 10.16766/j.cnki.issn.1674-4152.003205
Citation: ZHANG Zhen, ZHUO Feixiang, WU Yumeng, CHEN Xin. Expression and clinical significance of miR-374a-5p and RAB10 in cord blood of neonates with hypoxic-ischemic encephalopathy[J]. Chinese Journal of General Practice, 2023, 21(10): 1702-1705. doi: 10.16766/j.cnki.issn.1674-4152.003205
shu

Expression and clinical significance of miR-374a-5p and RAB10 in cord blood of neonates with hypoxic-ischemic encephalopathy

doi: 10.16766/j.cnki.issn.1674-4152.003205

  • Department of Pediatrics, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 KJ2021A0800

  • Received Date: 2022-11-20
    Available Online: 2023-11-23
    Abstract
  •   Objective  To investigate the expression levels and clinical significance of microRNA-374a-5p (miR-374a-5p) and RAB10 proteins in cord blood of neonates with hypoxic-ischemic encephalopathy (HIE).  Methods  A total of 75 newborns born in the First Affiliated Hospital of Bengbu Medical College from December 2020 to August 2022 were selected and divided into perinatal asphyxia group (26 cases) and HIE group (27 cases) according to the inclusion and exclusion criteria of HIE and whether the children had asphyxia. There were 14 cases in the mild HIE group, 7 cases in the moderate HIE group, 6 cases in the severe HIE group, and 22 cases in the healthy group. Umbilical cord blood was collected within 30 min after birth, and the expression levels of miR-374a-5p and RAB10 proteins were detected by real-time fluorescence quantification (qRT-PCR).  Results  There were no significant differences in gender, delivery mode, gestational age and birth weight among all groups (P>0.05). There were significant differences in miR-374a-5p (F=340.618, P < 0.001) and RAB10 (F=100.906, P < 0.001) among the asphyxia group, HIE group and healthy group. The expression level of serum miR-374a-5p in cord blood of neonates in HIE group was lower than that in healthy group, and RAB10 were higher than that in healthy group, the differences were statistically significant (all P < 0.05). The comparison of miR-374a-5p among the three groups with mild, moderate and severe HIE showed statistically significant differences (0.11±0.03, 0.08±0.03, 0.03±0.01, F=39.403, P < 0.001), the expression level of miR-374a-5p in moderate group was lower than that in mild group, and that in severe group was lower than that in mild group and moderate group, the differences were statistically significant (all P < 0.05). There was no significant difference in RAB10 level in cord blood among the three groups with mild, moderate and severe HIE (F=2.309, P=0.121).  Conclusion  The expression of miR-374a-5p expression is low in HIE children, which is related to the severity of the disease, the more severe the disease, the lower the expression level of miR-374a-5p, which can be used as a reference for early diagnosis and disease evaluation of HIE.

     

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