Volume 21 Issue 12
Dec.  2023
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Article Navigation >  Chinese Journal of General Practice  > 2023  >  21(12): 2040-2044
YANG Zhongyu, LI Wei, ZHU Guangzheng, ZHAO Wenyi, XU Jing. Effect of SFRP1 on the proliferation and invasion of head and neck squamous cell carcinoma and its mechanism[J]. Chinese Journal of General Practice, 2023, 21(12): 2040-2044. doi: 10.16766/j.cnki.issn.1674-4152.003285
Citation: YANG Zhongyu, LI Wei, ZHU Guangzheng, ZHAO Wenyi, XU Jing. Effect of SFRP1 on the proliferation and invasion of head and neck squamous cell carcinoma and its mechanism[J]. Chinese Journal of General Practice, 2023, 21(12): 2040-2044. doi: 10.16766/j.cnki.issn.1674-4152.003285
shu

Effect of SFRP1 on the proliferation and invasion of head and neck squamous cell carcinoma and its mechanism

doi: 10.16766/j.cnki.issn.1674-4152.003285
  • 1.

    Department of Plastic Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

 KJ2020A0576

 2020byzd137

 Byycxz21086

  • Received Date: 2023-02-11
    Available Online: 2024-01-29
    Abstract
  •   Objective  To observe the biological activities of head and neck squamous cell carcinoma (HNSC) in vitro by studying the proliferation, migration and mechanism of secreted frizzled related protein 1 (SFRP1), and to propose new therapeutic options for the treatment of HNSC.  Methods  The association of SFRP1 with head and neck squamous cell carcinoma was investigated using bioinformatics analysis of the correlation between SFRP1 expression levels in tumors and tumor invasion and proliferation. Skin cancer cells SCL-1 were knocked down the expression of SFRP1 using transfection, and the effect of cell proliferation, migration was examined using interference with SFRP1 group (si-SFRP1) and control group (si-NC) for CCK-8 assay analysis, scratch assay analysis and Transwell assay analysis, respectively. The Wnt/β-catenin signaling pathway and the expression levels of C-myc and cyclinD1 in SCL-1 cells were detected by Western blotting assay.  Results  Compared with the control head and neck squamous carcinoma cells, skin cancer cells were found to be low expressing SFRP1 protein (P < 0.010); the migration area ratio of the low expressing SFRP1 protein neck squamous cell carcinoma group was 0.602±0.019 at 24 hours, compared with 0.419±0.053 in the blank group and 0.435±0.009 in the control group, and the migration ability of the si-SFRP1 group was significantly higher than that of the control and blank groups (P < 0.01); in the Transwell experiment, the number of cells invaded by the si-SFRP1 group after 24 hours was 723.333±2.048, compared with 332.002±9.930 in the blank group and 343.332±32.504 in the control group, and the invasion ability of the si-SFRP1 group was significantly higher than that of the control and blank group (P < 0.01); knockdown of SFRP1 in SCL-1 was elevated through Wnt/β-catenin and downstream C-myc and cyclinD1 expression levels (P < 0.01). Statistical analysis of bioinformatics data revealed that knockdown of SFRP1 protein had promoted the proliferation of head and neck squamous cell carcinoma, which is a guide for clinical analysis.  Conclusion  SFRP1 is lowly expressed in skin cancer cells, and also promotes cell proliferation and migration ability through the Wnt/β-catenin signaling pathway.

     

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