Volume 22 Issue 8
Aug.  2024
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(8): 1317-1320
CHEN Xinru, HAN Rui, XU Jincheng. Fibulin-1 expression in tongue cancer and clinical value based on bioinformatics analysis[J]. Chinese Journal of General Practice, 2024, 22(8): 1317-1320. doi: 10.16766/j.cnki.issn.1674-4152.003625
Citation: CHEN Xinru, HAN Rui, XU Jincheng. Fibulin-1 expression in tongue cancer and clinical value based on bioinformatics analysis[J]. Chinese Journal of General Practice, 2024, 22(8): 1317-1320. doi: 10.16766/j.cnki.issn.1674-4152.003625
shu

Fibulin-1 expression in tongue cancer and clinical value based on bioinformatics analysis

doi: 10.16766/j.cnki.issn.1674-4152.003625

  • Department of Gastrointestinal Surgery, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233004, China

Funds:

 KJ2021ZD0088

 2021byzd112

  • Received Date: 2023-11-22
    Available Online: 2024-11-19
    Abstract
  •   Objective  To explore the expression and clinical significance of Fibulin-1 (FBLN1) in tongue cancer tissue and tongue cancer cells.  Methods  The expression of FBLN1 in head and neck cancer tissues was analyzed based on the HPA database. The association between FBLN1 and MKI67 expression in head and neck cancer was analyzed using the GEPIA database. The relationship between FBLN1 expression levels and the survival time of head and neck cancer patients was examined using the Kaplan-Meier Plotter database. Gene enrichment pathway prediction was performed to investigate the potential biological functions of FBLN1 in head and neck cancer. Immunohistochemistry (IHC) and Western blotting were used to validate the expression of FBLN1 in tongue cancer tissues. Stable overexpression of FBLN1 in tongue cancer cells was established, scratch and Transwell chamber experiments were conducted to assess its impact on cell invasion ability.  Results  Analysis from the HPA database showed that FBLN1 had lower expression levels in head and neck cancer tissues compared to adjacent non-cancerous tissues. Analysis from the GEPIA database revealed no significant difference (P>0.05) in FBLN1 expression between tumor tissues and adjacent non-cancerous tissues, while MKI67 exhibited higher expression levels (P < 0.05) with no correlation between their expressions (P=0.280). Survival analysis from Kaplan-Meier Plotter indicated no association between FBLN1 expression and overall survival time for tongue cancer patients (log-rank P=0.190), but higher survival rates were observed when FBLN1 was highly expressed (log-rank P=0.007). Gene enrichment pathways suggested that FBLN1 may be involved in multiple pathways related to tongue cancer development. IHC staining demonstrated that both staining intensity and positive area for Fibulin-1 were significantly higher than those observed for tumor tissue samples (P < 0.00l). Western blotting showed that the level of FBLN1 protein was higher in adjacent cancer tissues compared to tongue cancer tissues in 10 cases (P < 0.001). Scratch and Transwell experiments demonstrated that overexpression of FBLN1 significantly inhibits the migration and invasion ability of tongue squamous cell carcinoma cells Cal-27 (P < 0.05).  Conclusion  FBLN1 is downregulated in tongue cancer tissues, and overexpression of FBLN1 can suppress the invasive capability of Cal-27 cells, suggesting its potential as a prognostic marker for tongue cancer patients.

     

    • FullText(HTML)
  • loading
    • References(20)
  • [1]
    GUAN C, OUYANG D, QIAO Y, et al. CA9 transcriptional expression determines prognosis and tumour grade in tongue squamous cell carcinoma patients[J]. J Cell Mol Med, 2020, 24(10): 5832-5841. doi: 10.1111/jcmm.15252
    [2]
    HAEGGBLOM L, NASMAN A, RAMQVIST T, et al. TLR5 and TLR7 are differentially expressed in human papillomavirus-positive and negative base of tongue squamous cell carcinoma, and TLR7 may have an independent prognostic influence[J]. Acta Otolaryngol, 2019, 139(2): 206-210. doi: 10.1080/00016489.2018.1552014
    [3]
    JIA B, QIU X L, CHU H X, et al. Wnt7a predicts poor prognosis, and contributes to growth and metastasis in tongue squamous cell carcinoma[J]. Oncol Rep, 2019, 41(3): 1749-1758.
    [4]
    KINA S, NAKASONE T, KINJO T, et al. Outcomes after up-front surgery and metronomic neoadjuvant chemotherapy with S-1 or UFT for early tongue squamous cell carcinoma[J]. Clin Oral Invest, 2019, 23(6): 2593-2598. doi: 10.1007/s00784-018-2689-2
    [5]
    SHAO B B, HE L L. Hsa_circ_0001742 promotes tongue squamous cell carcinoma progression via modulating miR-634 expression[J]. Biochem Biophys Res Comm, 2019, 513(1): 135-140. doi: 10.1016/j.bbrc.2019.03.122
    [6]
    YANG X, ZHU J Y, DAI Y M, et al. Multi-parametric effect in predicting tumor histological grade by using susceptibility weighted magnetic resonance imaging in tongue squamous cell carcinoma[J]. BMC Med Imaging, 2019, 19(1): 24. DOI: 10.1186/s12880-019-0322-8.
    [7]
    MA Y, NENKOV M, SCHRÖDER D C, et al. Fibulin 2 is hypermethylated and suppresses tumor cell proliferation through inhibition of cell adhesion and extracellular matrix genes in non-small cell lung cancer[J]. Int J Mol Sci, 2021, 22(21): 11834. DOI: 10.3390/ijms222111834.
    [8]
    杨永锐, 王丽媛, 李海雯, 等. 灯盏乙素抑制NOX的表达改善非酒精性脂肪性肝病肝脏纤维化的研究[J]. 昆明医科大学学报, 2022, 43(7): 38-45.

    YANG Y R, WANG L Y, LI H W, et al. Scutellarin ameliorates liver fibrosis in nonalcoholic fatty liver disease by inhibiting NOX expression[J]. Journal of Kunming Medical University, 2022, 43(7): 38-45.
    [9]
    张清峰, 刘奎, 唐波. 两种手术方式治疗对非小细胞肺癌患者外周血肿瘤微转移指标的影响[J]. 贵州医科大学学报, 2021, 46(12): 1458-1462.

    ZHANG Q F, LIU K, TANG B, et al. Effects of two surgical methods on peripheral blood micro-metastasis indicators in patients with non-small cell lung cancer[J]. Journal of Guizhou Medical University, 2021, 46(12): 1458-1462.
    [10]
    唐嘉黛, 杨静, 宋红莉, 等. 318例原发性胃癌转移及预后影响因素的相关分析[J]. 昆明医科大学学报, 2023, 44(2): 75-87.

    TANG J D, YANG J, SONG H L, et al. Correlation of metastatic and prognostic factors in 318 cases of primary gastric cancer[J]. Journal of Kunming Medical University, 2023, 44(2): 75-87.
    [11]
    陈紫红, 孙士波, 王仙梅, 等. 早期结直肠癌患者凝血指标异常及其临床意义[J]. 昆明医科大学学报, 2021, 42(5): 96-100.

    CHEN Z H, SUN S B, WANG X M, et al. Abnormal coagulation parameters in patients with early colorectal cancer and its clinical significance[J]. Journal of Kunming Medical University, 2021, 42(5): 96-100.
    [12]
    HU H, LIANG L, ZHENG X, et al. Fibulin-1: a novel biomarker for predicting disease activity of the thyroid-associated ophthalmopathy[J]. Eye(Lond), 2023, 37(11): 2216-2219.
    [13]
    吴增波, 秘双燕, 晏燕. TNF-α在口腔鳞癌组织中的表达及临床意义[J]. 贵州医科大学学报, 2020, 45(9): 1047-1050, 1066.

    WU Z B, MI S Y, YAN Y. Expression and clinical significance of TNF-α in oral squamous cell carcinoma[J]. Journal of Guizhou Medical University, 2020, 45(9): 1047-1050, 1066.
    [14]
    马勇, 陈善勤, 傅军, 等. 血清纤维蛋白原及白蛋白/球蛋白比值水平与胃癌患者生存预后的关系[J]. 中华全科医学, 2023, 21(6): 945-949. doi: 10.16766/j.cnki.issn.1674-4152.003021

    MA Y, CHEN S Q, FU J, et al. Relationship between serum fibrinogen and albumin/globulin ratio levels and survival prognosis of patients with gastric cancer[J]. Chinese Journal of General Practice, 2023, 21(6): 945-949. doi: 10.16766/j.cnki.issn.1674-4152.003021
    [15]
    谭开宇, 刘丹, 刘华云, 等. COMPASS-CAT模型对妇科恶性肿瘤相关静脉血栓形成的风险预测[J]. 中华全科医学, 2021, 19(12): 2132-2135, 2140. doi: 10.16766/j.cnki.issn.1674-4152.002254

    TAN K Y, LIU D, LIU H Y, et al. Risk prediction of venous thromboembolism associated with gynaecologic cancer on the basis of the COMPASS-CAT risk assessment model[J]. Chinese Journal of General Practice, 2021, 19(12): 2132-2135, 2140. doi: 10.16766/j.cnki.issn.1674-4152.002254
    [16]
    曾益林, 袁帅, 唐岩, 等. 糖尿病合并乳腺癌患者超声引导置管感染后Smad信号通路和凝血因子的表达及意义[J]. 中华全科医学, 2020, 18(6): 1047-1050. doi: 10.16766/j.cnki.issn.1674-4152.001424

    ZENG Y L, YUAN S, TANG Y, et al. Expression and significance of Smad signal pathway and coagulation factor in patients with diabetes mellitus and breast cancer after ultrasound-guided catheter infection[J]. Chinese Journal of General Practice, 2020, 18(6): 1047-1050. doi: 10.16766/j.cnki.issn.1674-4152.001424
    [17]
    HARIKRISHNAN K, JOSHI O, MADANGIRIKAR S, et al. Cell derived matrix fibulin-1 associates with epidermal growth factor receptor to inhibit its activation, localization and function in lung cancer Calu-1 cells[J]. Front Cell Dev Biol, 2020, 8: 522. DOI: 10.3389/fcell.2020.00522.
    [18]
    WAN Y, SONG Y, CHEN J, et al. Upregulated fibulin-1 increased endometrial stromal cell viability and migration by repressing EFEMP1-dependent ferroptosis in endometriosis[J]. Biomed Res Int, 2022, 2022: 4809415. DOI: 10.1155/2022/4809415.
    [19]
    GONG J, JIE Y, XIAO C, et al. Increased expression of fibulin-1 is associated with hepatocellular carcinoma progression by regulating the Notch signaling pathway[J]. Front Cell Dev Biol, 2020, 8: 478. DOI: 10.3389/fcell.2020.00478.
    [20]
    YANG F, SHI J, ZHANG L, et al. Role of fibulin-1 gene promoter methylation in the carcinogenesis and development of tongue squamous cell carcinoma[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2022, 133(4): 432-440.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(2)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (1) PDF downloads(0) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return