Volume 22 Issue 11
Nov.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(11): 1822-1826
YU Jinhui, WANG Wei, LYU Zhenyu, JI Wenbin, YU Huihao, YANG Yan. Comprehensive analysis of the prognostic and immunological significance of EXO1 in hepatocellular carcinoma[J]. Chinese Journal of General Practice, 2024, 22(11): 1822-1826. doi: 10.16766/j.cnki.issn.1674-4152.003742
Citation: YU Jinhui, WANG Wei, LYU Zhenyu, JI Wenbin, YU Huihao, YANG Yan. Comprehensive analysis of the prognostic and immunological significance of EXO1 in hepatocellular carcinoma[J]. Chinese Journal of General Practice, 2024, 22(11): 1822-1826. doi: 10.16766/j.cnki.issn.1674-4152.003742
shu

Comprehensive analysis of the prognostic and immunological significance of EXO1 in hepatocellular carcinoma

doi: 10.16766/j.cnki.issn.1674-4152.003742

  • Department of Medical Oncology, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233004, China

Funds:

 2023AH040291

 gxyq2022042

 Byycx23058

  • Received Date: 2024-05-11
    Available Online: 2024-12-31
    Abstract
  •   Objective  To analyze the expression of exonuclease 1 (EXO1) in hepatocellular carcinoma (HCC) and to investigate the relationship between EXO1 and prognosis and immune cell infiltration in patients with HCC.  Methods  The expression of EXO1 in HCC and normal tissues was analyzed based on The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases, Furthermore, EXO1 mRNA expression was verified in HCC cell lines by qRT-PCR analysis. The correlation between EXO1 expression and clinicopathological features was analyzed using logistic regression. Kaplan-Meier analysis and Cox regression were used to determine the correlation between EXO1 expression and patients prognosis. The GSVA ssGSEA algorithm and the TIMER database were employed to examine the correlation between EXO1 expression and the level of immune infiltration.  Results  The expression of EXO1 mRNA in HCC tissues was found to be significantly higher than that in normal tissues (P < 0.001). Furthermore, the EXO1 mRNA level in HCC cell lines, BEL-7404 and SMMC-7721 was observed to be markedly elevated in comparison to that in a normal hepatic cell line LO2 (3.92±0.75, 2.42±0.52 vs. 1.00±0.00; P < 0.05). There was a significant correlation between EXO1 expression and the T stage, histological grade and pathological stage of tumors (P < 0.001). A high level of EXO1 expression was found to be associated with poorer overall survival rate in HCC patients (P < 0.001). The analysis of immune infiltration revealed a correlation between EXO1 expression and the infiltration of multiple immune cells.  Conclusion  High EXO1 expression is associated with an unfavourable prognosis and immune cell infiltration in HCC patients. This may serve as a potential prognostic biomarker and immune intervention target in HCC.

     

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    • References(15)
  • [1]
    ZHANG C H, CHENG Y F, ZHANG S, et al. Changing epidemiology of hepatocellular carcinoma in Asia[J]. Liver Int, 2022, 42(9): 2029-2041. doi: 10.1111/liv.15251
    [2]
    汪蕊, 陈宇佛, 张甜甜, 等. 卡瑞利珠单抗联合阿帕替尼对晚期肝癌患者健康相关生活质量的影响[J]. 中华全科医学, 2023, 21(8): 1295-1298. doi: 10.16766/j.cnki.issn.1674-4152.003106

    WANG R, CHEN Y F, ZHANG T T, et al. Effect of camrelizumab in combination with apatinib on health-related quality of life in patients with advanced liver cancer[J]. Chinese Journal of General Practice, 2023, 21(8): 1295-1298. doi: 10.16766/j.cnki.issn.1674-4152.003106
    [3]
    吴珊, 邱俊, 陈俊宇, 等. EXO1蛋白在DNA损伤中研究进展[J]. 中国老年学杂志, 2022, 42(5): 1259-1262.

    WU S, QIU J, CHEN J Y, et al. Research progress of EXO1 protein in DNA damage[J]. Chinese Journal of Gerontology, 2022, 42(5): 1259-1262.
    [4]
    YANG G, DONG K S, ZHANG Z Y, et al. EXO1 plays a carcinogenic role in hepatocellular carcinoma and is related to the regulation of FOXP3[J]. J Cancer, 2020, 11(16): 4917-4932. doi: 10.7150/jca.40673
    [5]
    MA J X, JIN J P, LU H S, et al. Exonuclease 1 is a potential diagnostic and prognostic biomarker in hepatocellular carcinoma[J]. Front Mol Biosci, 2022, 9: 889414. DOI: 10.3389/fmolb.2022.889414.
    [6]
    CHEN C, WANG Z H, DING Y, et al. Tumor microenvironment-mediated immune evasion in hepatocellular carcinoma[J]. Front Immunol, 2023, 14: 1133308. DOI: 10.3389/fimmu.2023.1133308.
    [7]
    CHENG Q Q, WANG W, LIU J, et al. Elevated MPP6 expression correlates with an unfavorable prognosis, angiogenesis and immune evasion in hepatocellular carcinoma[J]. Front Immunol, 2023, 14: 1173848. DOI: 10.3389/fimmu.2023.1173848.
    [8]
    BARBIE D A, TAMAYO P, BOEHM J S, et al. Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1[J]. Nature, 2009, 462(7269): 108-112. doi: 10.1038/nature08460
    [9]
    ZHAO L, ZANG Q, LIANG G D, et al. LncRNA CECR7 boosts hepatocellular carcinoma progression by recruiting RNA binding protein U2AF2 to enhance the stability of EXO1 mRNA[J]. Heliyon, 2023, 9(9): e19862. DOI: 10.1016/j.heliyon.2023.e19862.
    [10]
    ZHANG L M, JIANG B W, LAN Z X, et al. Immune infiltration landscape on prognosis and therapeutic response and relevant epigenetic and transcriptomic mechanisms in lung adenocarcinoma[J]. Front Immunol, 2022, 13: 983570. DOI: 10.3389/fimmu.2022.983570.
    [11]
    BLAGIH J, BUCK M D, VOUSDEN K H. P53, cancer and the immune response[J]. J Cell Sci, 2020, 133(5): jcs237453. DOI: 10.1242/jcs.237453.
    [12]
    于栋华, 王晓菲, 付佳琪, 等. 中性粒细胞胞外诱捕网在疾病中的作用及中药干预的研究进展[J]. 中国医药, 2022, 17(5): 793-797.

    YU D H, WANG X F, FU J Q, et al. The role of neutrophil extracellular trapping net in disease and the research progress of Chinese medicine intervention[J]. Chinese Medicine, 2022, 17(5): 793-797.
    [13]
    HEDRICK C C, MALANCHI I. Neutrophils in cancer: heterogeneous and multifaceted[J]. Nat Rev Immunol, 2022, 22(3): 173-187. doi: 10.1038/s41577-021-00571-6
    [14]
    CHENG K, CAI N, ZHU J H, et al. Tumor-associated macrophages in liver cancer: from mechanisms to therapy[J]. Cancer Commun(Lond), 2022, 42(11): 1112-1140.
    [15]
    LI T H, LIU T Y, ZHU W J, et al. Targeting MDSC for immune-checkpoint blockade in cancer immunotherapy: current progress and new prospects[J]. Clin Med Insights Oncol, 2021, 15: 11795549211035540. DOI: 10.1177/11795549211035540.
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