Volume 22 Issue 11
Nov.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(11): 1842-1845
WU Kangping, HUANG Jing, WANG Lina, YE Bei, ZHU Yanan. The expression and clinical significance of PTEN and miR-215 in retinoblastoma[J]. Chinese Journal of General Practice, 2024, 22(11): 1842-1845. doi: 10.16766/j.cnki.issn.1674-4152.003746
Citation: WU Kangping, HUANG Jing, WANG Lina, YE Bei, ZHU Yanan. The expression and clinical significance of PTEN and miR-215 in retinoblastoma[J]. Chinese Journal of General Practice, 2024, 22(11): 1842-1845. doi: 10.16766/j.cnki.issn.1674-4152.003746
shu

The expression and clinical significance of PTEN and miR-215 in retinoblastoma

doi: 10.16766/j.cnki.issn.1674-4152.003746

  • Department of Pediatric Respiratory, Hangzhou Children' s Hospital, Hangzhou, Zhejiang 310002, China

Funds:

 81970779

  • Received Date: 2024-03-19
    Available Online: 2024-12-31
    Abstract
  •   Objective  Retinoblastoma (RB) can impair the vision and threaten the lives of children. The purpose of this study is to provide guidance for individualized treatment and prognosis assessment of children by investigating the expression and clinical significance of phosphatase and tensin homolog deleted on chromosome ten (PTEN) and miR-215 in RB.  Methods  A total of 80 children with RB, diagnosed and treated in Hangzhou Children ' s Hospital from August 2020 to October 2023 were selected for the study. Cancerous tissues and adjacent normal tissues were collected, with miR-215 levels detected by RT-PCR and PTEN expression evaluated by immunohistochemical method, respectively. Cox risk regression model was used to analyze the relationship among the expression of PTEN, miR-215, pathological parameters and prognosis of RB.  Results  The positive rate of PTEN [65.00% (52/80) vs. 100.00% (80/80)] and the expression level of miR-215 in RB tissues were significantly lower than those in adjacent normal tissues (P < 0.05). The overall survival (OS) was significantly reduced in both the PTEN-negative expression group and the OS in miR-215 low-expression group (P < 0.05). Cox regression analysis showed that stage Ⅲ (HR=3.047, 95% CI: 1.117-8.311, P=0.030), undifferentiated tumors (HR=2.907, 95% CI: 1.452-5.817, P=0.003), optic nerve infiltration (HR=4.446, 95% CI: 1.311-15.076, P=0.017), low expression of miR-215 (HR=2.854, 95% CI: 1.211-6.604, P=0.018), and negative expression of PTEN (HR=3.823, 95% CI: 1.708-8.555, P=0.001) as independent risk factors for poor prognosis in RB children (P < 0.05).  Conclusion  The decrease of positive rate of PTEN and low expression of miR-215 are observed in RB children, and these factors are risk factors associated with poor prognosis of RB.

     

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