Volume 22 Issue 11
Nov.  2024
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Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(11): 1854-1858
WANG Haiquan, LI Zhaohui, SUN Shengan, WANG Yunshuai, SHI Shousen. Research on the regulation of malignant biological behavior of colon cancer cells by Hsa miR-654-5p and its clinical significance[J]. Chinese Journal of General Practice, 2024, 22(11): 1854-1858. doi: 10.16766/j.cnki.issn.1674-4152.003749
Citation: WANG Haiquan, LI Zhaohui, SUN Shengan, WANG Yunshuai, SHI Shousen. Research on the regulation of malignant biological behavior of colon cancer cells by Hsa miR-654-5p and its clinical significance[J]. Chinese Journal of General Practice, 2024, 22(11): 1854-1858. doi: 10.16766/j.cnki.issn.1674-4152.003749
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Research on the regulation of malignant biological behavior of colon cancer cells by Hsa miR-654-5p and its clinical significance

doi: 10.16766/j.cnki.issn.1674-4152.003749

  • Gastroenterology Department of Luoyang Central Hospital, Luoyang, Henan 471000, China

Funds:

 LHGJ20220939

 LHGJ20220940

  • Received Date: 2023-11-24
    Available Online: 2024-12-31
    Abstract
  •   Objective  Detect the expression of Hsa-miR-654-5p in colon cancer, analyze its impact on the malignant biological behavior of colon cancer cells and its clinical significance.  Methods  Detect the expression of Hsa-miR-654-5p in colon cancer cells (HT-29, Caco-2, SW480, HCT116, LOVO) and human normal colon epithelial cell (HCoEpiC), as well as in 90 cases of colon cancer and adjacent tissues collected from the central laboratory of Luoyang Central Hospital from January 2020 to December 2022 by qRT-PCR, analyze the relationship between its expression and clinical pathological characteristics and prognosis of patients. Conduct experimental grouping according to the simple random grouping method: NC group, miR-con group, Hsa-miR-654-5p overexpression group and knockdown group (n=10 per group). Cell proliferation, cloning, migration, and invasion changes in each group were measured by thiazole blue colorimetry, clone formation assay, cell scratch assay, and Transwell assay, detected the expression of Epithelia cadherin, human vimentin, and Nervous cadheri by Western blotting.  Results  The expression level of Hsa-miR-654-5p was significantly increased in colon cancer cells and tissues. There were significant differences in tumor size, differentiation, TNM staging, and lymph node metastasis (P < 0.05), while there were no statistically significant differences in age, gender, pathological type, and carcinoembryonic antigen (P>0.05). The proliferation and clone ability, cell migration rate, number of transmembrane cells, and protein expression of Epithelia cadherin, human vimentin, and Nervous cadherin of SW480 cells in the Hsa-miR-654-5p overexpression group were significantly increased when compared with the NC group and miR-con group, while the knockdown group showed a significant decrease in the above indicators, with significant differences between groups (P < 0.05).The median survival of colon cancer patients with high expression of Hsa-miR-654-5p was significantly lower than those with low expression (20.44 months vs. 38.16 months, χ2=10.079, P < 0.001).  Conclusion  Hsa-miR-654-5p is highly expressed in colon cancer and significantly correlated with clinicopathological features and prognosis of patients. Knockdown of it can inhibit the malignant biological behavior of colon cancer cells, which may be related to the inhibition of epithelial-mesenchymal transition.

     

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