Volume 22 Issue 12
Dec.  2024
Turn off MathJax
Article Contents
Article Navigation >  Chinese Journal of General Practice  > 2024  >  22(12): 2049-2052
CUI Shuling, LAO Peiwei, SHEN Kewei, ZHANG Yingzhi. The role of estrogen-induced DCN gene methylation in the pathogenesis of pelvic organ prolapse[J]. Chinese Journal of General Practice, 2024, 22(12): 2049-2052. doi: 10.16766/j.cnki.issn.1674-4152.003795
Citation: CUI Shuling, LAO Peiwei, SHEN Kewei, ZHANG Yingzhi. The role of estrogen-induced DCN gene methylation in the pathogenesis of pelvic organ prolapse[J]. Chinese Journal of General Practice, 2024, 22(12): 2049-2052. doi: 10.16766/j.cnki.issn.1674-4152.003795
shu

The role of estrogen-induced DCN gene methylation in the pathogenesis of pelvic organ prolapse

doi: 10.16766/j.cnki.issn.1674-4152.003795

  • Department of Gynaecology, the First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China

Funds:

 2023KY1106

  • Received Date: 2024-02-25
    Available Online: 2025-01-20
    Abstract
  •   Objective  To detect the methylation of estradiol (E2) and decorin (DCN) genes in patients with pelvic organ prolapse (POP) and to study their role in the pathogenesis of POP.  Methods  A total of 85 POP patients who underwent surgery between January 2023 and January 2024 were selected for the postmenopause group (PSM group, n=44) and menopausal transition period group (TRM group, n=41), additionally, 30 patients with pelvic benign diseases during the same period were selected as the control group. The Primary fibroblasts were treated with 50 nmol/L E2 (H-EST group), 2 nmol/L E2 (L-EST group), and 50 nmol/L E2 in combination with an ER antagonist (ESR siRNA, H-EST+siER group). Detect and compare the methylation differences of the estradiol (E2), DCN, type Ⅰ collagenase (COL Ⅰ), E2 receptor (ER) and DCN genes.  Results  The expressions of E2, DCN, COL Ⅰ and ER in the PSM group were significantly lower than those in the TRM group and the control group (P < 0.05), Furthermore the DCN methylation rate was significantly higher in the PSM group than in the TRM group and the control group (P < 0.05). In patients with POP in the PSM group and TRM group, E2 was found to be positively correlated with the expression of DCN, COL Ⅰ, and ER (rs=0.713, 0.654, 0.679, 0.639, respectively, all P < 0.05), and negatively correlated with DCN gene methylation (rs=-0.821, -0.806, all P < 0.05). The expressions of DCN, COL Ⅰ and ER in cells of the H-EST group and L-EST group were significantly lower than those of the CON group, H-EST+siER group and siER group (P < 0.05). Furthermore, the DCN methylation rate was significantly higher than that of the CON group, H-EST+siER group and siER group (P < 0.05).  Conclusion  The methylation rate of DCN genes and E2 level is markedly elevated in patients with POP, and there is a negative correlation. E2 may play a role in the development of POP by inducing methylation of the DCN gene.

     

    • FullText(HTML)
  • loading
    • References(25)
  • [1]
    COLLINS S, LEWICKY-GAUPP C. Pelvic organ prolapse[J]. Gastroenterol Clin North Am, 2022, 51(1): 177-193. doi: 10.1016/j.gtc.2021.10.011
    [2]
    王琦, 程贤鹦. 初产妇产后早期盆底功能障碍性疾病与分娩相关因素分析[J]. 中华全科医学, 2022, 20(1): 76-79. doi: 10.16766/j.cnki.issn.1674-4152.002281

    WANG Q, CHENG X Y. Analysis between postpartum pelvic floor dysfunction and delivery related factors in primipara[J]. Chinese Journal of General Practice, 2022, 20(1): 76-79. doi: 10.16766/j.cnki.issn.1674-4152.002281
    [3]
    PIZZOFERRATO A C, THUILLIER C, VÉNARA A, et al. Management of female pelvic organ prolapse-summary of the 2021 HAS guidelines[J]. J Gynecol Obstet Hum Reprod, 2023, 52(3): 102535. DOI: 10.1016/j.jogoh.2023.102535.
    [4]
    胡凌波, 潘程瑶, 杨素娟, 等. 基于信息-动机-行为技巧模式的康复训练在产后盆底肌修复中的应用[J]. 中华全科医学, 2023, 21(11): 1946-1950. doi: 10.16766/j.cnki.issn.1674-4152.003264

    HU L B, PAN C Y, YANG S J, et al. Application of rehabilitation training based on information-motivation-behavioral skills model in postpartum pelvic floor muscle repair[J]. Chinese Journal of General Practice, 2023, 21(11): 1946-1950. doi: 10.16766/j.cnki.issn.1674-4152.003264
    [5]
    宋新玲, 刘丽丽. 盆底神经肌肉刺激联合普罗雌烯阴道用软胶囊对子宫全切术后患者盆底功能恢复的治疗效果分析[J]. 中国妇幼保健, 2024, 39(4): 610-613.

    SONG X L, LIU L L. Analysis of the therapeutic effect of pelvic floor neuromuscular stimulation combined with proestren vaginal soft capsules on the recovery of pelvic floor function in patients after total hysterectomy[J]. Chinese Maternal and Child Health Care, 2024, 39(4): 610-613.
    [6]
    SCHULTEN S F M, CLAAS-QUAX M J, WEEMHOFF M, et al. Risk factors for primary pelvic organ prolapse and prolapse recurrence: an updated systematic review and meta-analysis[J]. Am J Obstet Gynecol, 2022, 227(2): 192-208. doi: 10.1016/j.ajog.2022.04.046
    [7]
    ISALI I, ABDELDAYEM J, EL-NASHAR S. Gene expression in urinary incontinence and pelvic organ prolapse: a review of literature[J]. Curr Opin Obstet Gynecol, 2020, 32(6): 441-448. doi: 10.1097/GCO.0000000000000661
    [8]
    KOVÁCS T, SZABÓ-MELEG E, ÁBRAHÁM I M. Estradiol-induced epigenetically mediated mechanisms and regulation of gene expression[J]. Int J Mol Sci, 2020, 21(9): 3177. DOI: 10.3390/ijms21093177.
    [9]
    MUHAMMAD J S, GUIMEI M, JAYAKUMAR M N, et al. Estrogen-induced hypomethylation and overexpression of YAP1 facilitate breast cancer cell growth and survival[J]. Neoplasia, 2021, 23(1): 68-79. doi: 10.1016/j.neo.2020.11.002
    [10]
    中华医学会妇产科学分会妇科盆底学组. 盆腔器官脱垂的中国诊治指南(2020年版)[J]. 中华妇产科杂志, 2020, 55(5): 300-306.

    Gynecological Pelvic Floor Group of the Obstetrics and Gynecology Branch of the Chinese Medical Association. Chinese guidelines for diagnosis and treatment of pelvic organ prolapse (2020 edition)[J]. Chinese Journal of Obstetrics and Gynecology, 2020, 55(5): 300-306.
    [11]
    胡烨, 张永莉, 杜贵强. 机械应力对人子宫旁韧带成纤维细胞增殖及胶原代谢的影响[J]. 中国生育健康杂志, 2023, 34(4): 307-312.

    HU Y, ZHANG Y L, DU G Q. Effects of mechanical stress on proliferation and collagen metabolism of female parauterine ligament fibroblasts[J]. Chinese Journal of Reproductive Health, 2023, 34(4): 307-312.
    [12]
    YU X, HE L, WANG Y, et al. Local estrogen therapy for pelvic organ prolapse in postmenopausal women: a systematic review and meta-analysis[J]. Iran J Public Health, 2022, 51(8): 1728-1740.
    [13]
    WEINTRAUB A Y, GLINTER H, MARCUS-BRAUN N. Narrative review of the epidemiology, diagnosis and pathophysiology of pelvic organ prolapse[J]. Int Braz J Urol, 2020, 46(1): 5-14.
    [14]
    XIE T, GUO D, GUO T, et al. The protective effect of 17β-estradiol on human uterosacral ligament fibroblasts from postmenopausal women with pelvic organ prolapse[J]. Front Physiol, 2022, 13: 980843. DOI: 10.3389/fphys.2022.980843.
    [15]
    REDDY R A, CORTESSIS V, DANCZ C, et al. Role of sex steroid hormones in pelvic organ prolapse[J]. Menopause, 2020, 27(8): 941-951.
    [16]
    冉津川, 胡静, 陈炳力, 等. 盆底功能障碍中胶原蛋白代谢机制及电针对胶原蛋白的调节[J]. 上海针灸杂志, 2022, 41(4): 413-417.

    RAN J C, HU J, CHEN B L, et al. Collagen metabolism mechanism and regulation of collagen by electroacupuncture in pelvic floor dysfunction[J]. Shanghai Journal of Acupuncture, 2022, 41(4): 413-417.
    [17]
    王翮, 平毅. TGFBR2、TGF-β1及CTGF在盆腔器官脱垂患者子宫韧带组织中的表达及意义[J]. 山西医科大学学报, 2023, 54(8): 1154-1160.

    WANG H, PING Y. Expression and significance of TGFBR2, TGF-β1, and CTGF in uterine ligament tissue of patients with pelvic organ prolapse[J]. Journal of Shanxi Medical University, 2023, 54(8): 1154-1160.
    [18]
    ROMAN B, KUMAR S A, ALLEN S C, et al. A model for studying the biomechanical effects of varying ratios of collagen types Ⅰ and Ⅲ on cardiomyocytes[J]. Cardiovasc Eng Technol, 2021, 12(3): 311-324.
    [19]
    哈提古丽·尼斯尔, 祖木热来提·艾尼瓦尔, 米合日尼沙·买买提, 等. 不同群体女性盆底功能障碍性疾病患者盆底组织中胶原蛋白Ⅰ、Ⅲ表达的研究[J]. 中国生育健康杂志, 2022, 33(5): 459-462, 473.

    KHATIGUL NISIR, ZUMURETI ANIWAL, MIHEZHINISHA MAIMAITI, et al. Expression of collagen Ⅰ and Ⅲ in pelvic floor tissues of different groups of female patients with pelvic floor dysfunction diseases research[J]. Chinese Journal of Reproductive Health, 2022, 33(5): 459-462, 473.
    [20]
    赵茂宇, 付静, 熊秋璨, 等. 核心蛋白聚糖对高糖诱导心肌细胞丝裂原活化蛋白激酶信号通路及纤维化的影响[J]. 中华老年心脑血管病杂志, 2023, 25(7): 751-755.

    ZHAO M Y, FU J, XIONG Q C, et al. Effects of decorin on mitogen-activate protein kinase signaling pathway and fibrosis of cardiomyocytes induced by high glucose[J]. Chinese Journal of Geriatric Heart Brain and Vessel Diseases, 2023, 25(7): 751-755.
    [21]
    姜晖. 核心蛋白聚糖在瘢痕疙瘩发生过程中的作用及机制研究[D]. 延吉: 延边大学, 2024.

    JIANG H. Research on the role and mechanism of decorin in the occurrence of keloids[D]. Yanji: Yanbian University, 2024.
    [22]
    LI X J, PAN H T, CHEN J J, et al. Proteomics of uterosacral ligament connective tissue from women with and without pelvic organ prolapse[J]. Proteomics Clin Appl, 2019, 13(4): e1800086. DOI: 10.1002/prca.201800086.
    [23]
    GARDINI E S, FIACCO S, MERNONE L, et al. Sleep and methylation of estrogen receptor genes, ESR1 and GPER, in healthy middle-aged and older women: findings from the women 40+ healthy aging study[J]. Nat Sci Sleep, 2020, 12: 525-536.
    [24]
    黄一波, 牛英群, 黄国强, 等. 基于时钟基因启动子区甲基化的胃癌患者预后预测模型的建立与验证[J]. 中华全科医学, 2022, 20(1): 43-46. doi: 10.16766/j.cnki.issn.1674-4152.002272

    HUANG Y B, NIU Y Q, HUANG G Q, et al. Establishment and verification of a prognostic model for patients with gastric cancer based on clock gene promoter methylation[J]. Chinese Journal of General Practice, 2022, 20(1): 43-46. doi: 10.16766/j.cnki.issn.1674-4152.002272
    [25]
    RESZEGI A, HORVÁTH Z, FEHÉR H, et al. Protective role of decorin in primary hepatocellular carcinoma[J]. Front Oncol, 2020, 10: 645. DOI: 10.3389/fonc.2020.00645.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(1)  / Tables(4)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (10) PDF downloads(2) Cited by()
    Proportional views
    Related

    Address：No. 287, Changhuai Road, Bengbu, Anhui Province, 233004, P.R.ChinaphoneNo：0552-3066635； 0552-3051890Email：zhqkyx@163.com

    Copyright © Chinese Journal of General Practice皖ICP备2020018345号-2

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return