Volume 23 Issue 2
Feb.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(2): 219-222
REN Ying, HE Yu, LI Ping. A study on the correlation between FGFR3 and TERT gene mutations and the pathological grade of urethral epithelial carcinoma[J]. Chinese Journal of General Practice, 2025, 23(2): 219-222. doi: 10.16766/j.cnki.issn.1674-4152.003871
Citation: REN Ying, HE Yu, LI Ping. A study on the correlation between FGFR3 and TERT gene mutations and the pathological grade of urethral epithelial carcinoma[J]. Chinese Journal of General Practice, 2025, 23(2): 219-222. doi: 10.16766/j.cnki.issn.1674-4152.003871
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A study on the correlation between FGFR3 and TERT gene mutations and the pathological grade of urethral epithelial carcinoma

doi: 10.16766/j.cnki.issn.1674-4152.003871

  • Department of Pathology, the Affiliated People ' s Hospital of Ningbo University, Ningbo, Zhejiang 315040, China

Funds:

 2023KY1146

  • Received Date: 2024-03-18
    Available Online: 2025-03-27
    Abstract
  •   Objective  To investigate the correlation between FGFR3and TERT gene mutations and the pathological grade of urethral epithelial carcinoma, aims to provide some reference for exploring the publication mechanism of the disease.  Methods  A total of 150 patients with urothelial carcinoma hospitalized in the department of Urology at the Affiliated People's Hospital of Ningbo University from January 2021 to March 2023 were selected as the study subjects.Fifteen healthy subjects were selected as the control group.Genomic DNA was extracted, and FGFR3 and TERT gene polymorphisms were analyzed by real-time PCR.The relationship between the cytopathological diagnostic grade was explored.  Results  The DNA content of FGFR3 exons 7, 10, and 15 was higher in the experimental group than the control group (P < 0.05).The HIT group (T1, T2, T3, T4, and Tis) showed higher DNA content than the LIT group (Ta, P < 0.05).The DNA content of FGFR3 exons 7, 10, and 15 increased gradually with rising tumor malignancy in the experimental group.For the TERT promoter, the proportion of wild type in C228T and C250T polymorphisms decreased as tumor malignancy increased: Ta (5/8, 62.5%), T1(11/29, 37.9%), T2(9/40, 22.5%), T3(7/28, 25.0%), T4(7/27, 25.9%), and Tis (4/18, 22.2%).The HIT group (T1, T2, T3, T4 and Tis) showed C228T mutations, C250T mutations, and C228T+C250T double mutations.  Conclusion  The DNA content of FGFR3 exons 7, 10, and 15, along with the C228T and C250T polymorphisms of the TERT promoter, correlates with the pathological grade of urothelial carcinoma pathological grade.These markers can be used as biomarkers for assessing pathological grade of urothelial carcinoma.

     

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