Volume 23 Issue 7
Jul.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(7): 1135-1139
CHEN Tingting, ZHU Yongna, WU Yue, LIU Xi. The regulation of METTL3 on the proliferation and migration of SCAPs in the inflammatory environment[J]. Chinese Journal of General Practice, 2025, 23(7): 1135-1139. doi: 10.16766/j.cnki.issn.1674-4152.004082
Citation: CHEN Tingting, ZHU Yongna, WU Yue, LIU Xi. The regulation of METTL3 on the proliferation and migration of SCAPs in the inflammatory environment[J]. Chinese Journal of General Practice, 2025, 23(7): 1135-1139. doi: 10.16766/j.cnki.issn.1674-4152.004082
shu

The regulation of METTL3 on the proliferation and migration of SCAPs in the inflammatory environment

doi: 10.16766/j.cnki.issn.1674-4152.004082
  • 1.

    Department of Stomatology, the Second Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233040, China

Funds:

 2023AH051997

  • Received Date: 2024-11-05
    Available Online: 2025-10-25
    Abstract
  •   Objective  To explore whether METTL3 plays a regulatory role in the proliferation and migration capacity of apical dental papilla stem cells (SCAPs) in inflammatory environments and to provide a new theoretical basis for clinical endodontic regenerative therapy.  Methods  A permanent tooth apical periodontitis model of young rat was constructed by means of the pulp exposure method. In addition, AP-SCAPs and H-SCAPs were isolated, cultured and identified. The proliferation and migration potential of the cells was detected by CCK-8 and scratch assay, and the expression level of METTL3 was detected by Q-PCR in the two groups. Lentiviral transfection was utilized to construct AP-SCAPs overex-pressing METTL3 (Lv-M), with Lv-M-Ctrl serving as the control. The construction of AP-SCAPs silencing METTL3 (si-M) was achieved by employing the group. siRNA interference method. The proliferation and migration ability of cells in each group was detected by CCK-8 and scratch assay, respectively.  Results  In comparison with H-SCAPs, AP-SCAPs demonstrated a reduced capacity for proliferation and migration (P < 0.05). Furthermore, and the METTL3 expression levels in AP-SCAPs were significantly lower than those in H-SCAPs (1.00±0.05 vs. 1.62±0.17, P=0.004). The CCK-8 results demonstrated that the proliferation capacity of Lv-M was elevated at 24, 72, 120 and 168 hours in comparison to the control group (P < 0.05). In comparison with the si-M-Ctrl group, the si-M group demonstrated a decrease in cell proliferation ability (P < 0.05). The results of the scratch assay demonstrated that the cell migration rate of the Lv-M group was higher than that of the Lv-M-Ctrl group [(70.21±3.66)% vs. (27.26±4.69)%, P < 0.001]. Furthermore, the cell migration rate of the si-M group was decreased in comparison with the si-M-Ctrl group [(44.11±3.90)% vs. (72.33±5.61)%, P=0.002].  Conclusion  METTL3 has been demonstrated to play a regulatory role in the proliferation and migration of SCAPs within an inflammatory environment.

     

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