Volume 23 Issue 11
Nov.  2025
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Article Navigation >  Chinese Journal of General Practice  > 2025  >  23(11): 1874-1877
HU Kunmiao, ZHOU Limin, YU Zhenhua. Characteristics of gastric microbiota in patients with drug-resistant Helicobacter pylori infection[J]. Chinese Journal of General Practice, 2025, 23(11): 1874-1877. doi: 10.16766/j.cnki.issn.1674-4152.004249
Citation: HU Kunmiao, ZHOU Limin, YU Zhenhua. Characteristics of gastric microbiota in patients with drug-resistant Helicobacter pylori infection[J]. Chinese Journal of General Practice, 2025, 23(11): 1874-1877. doi: 10.16766/j.cnki.issn.1674-4152.004249
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Characteristics of gastric microbiota in patients with drug-resistant Helicobacter pylori infection

doi: 10.16766/j.cnki.issn.1674-4152.004249

  • Department of Gastroenterology, Chun' an Branch of Zhejiang Provincial People' s Hospital, Hangzhou, Zhejiang 311700, China

Funds:

 2022KY1073

  • Received Date: 2025-02-14
    Available Online: 2026-01-07
    Abstract
  •   Objective  Helicobacter pylori (H. pylori) infection is closely related to the occurrence and development of various gastric diseases, such as chronic gastritis, peptic ulcer disease, and gastric cancer. However, its mechanisms of drug resistance are complex, and the failure rate of traditional eradication programs remains high. Emerging evidence suggests that the gastric microflora may affect H. pylori drug resistance by regulating the local microenvironment or mediating antibiotic metabolism. This study aimed to analyze the characteristics of the gastric bacterial flora in patients with drug-resistant H. pylori infection.  Methods  A total of 184 patients with Helicobacter pylori infection admitted to Chun' an Branch of Zhejiang Provincial People' s hospital from January 2023 to June 2024 were selected as the study objects, and all of them were tested for drug sensitivity. According to the results of drug susceptibility test, the patients were divided into the single-drug resistance group, the double-drug resistance group, and the multiple-drug resistance group. Gastric tissue samples were collected from all patients, and the characteristics of gastric microbiota were detected based on 16srRNA sequencing, including α diversity (Simpson index and Shannon index) and β diversity (based on principal component analysis). LEfSe analysis was used to identify bacteria genera with significant differences among all groups.  Results  Significant differences were observed in the alpha-diversity indices (Shannon and Simpson) among the three patient groups (P < 0.05). The mono-resistant group exhibited the lowest Shannon index (2.54±0.45) and the highest Simpson index (0.67±0.15), whereas the multidrug-resistant group demonstrated the highest Shannon index (4.26±0.34) and the lowest Simpson index (0.44±0.11), P < 0.05. Principal component analysis revealed considerable diversity disparities among the groups (35.6% vs. 27.8%).  Conclusion  The gastric microbiota composition and diversity differ significantly among patients with varying degrees of H. pylori drug resistance.

     

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