Volume 24 Issue 2
Feb.  2026
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Article Navigation >  Chinese Journal of General Practice  > 2026  >  24(2): 200-204
LI Zhixin, LYU Tie, JIN Liezhou, CHEN Lyujian, YING Lijun. Effects of continuous blood purification therapy on mitochondrial DNA and mitochondrial complex Ⅴ in peripheral blood mononuclear cells of sepsis patients[J]. Chinese Journal of General Practice, 2026, 24(2): 200-204. doi: 10.16766/j.cnki.issn.1674-4152.004360
Citation: LI Zhixin, LYU Tie, JIN Liezhou, CHEN Lyujian, YING Lijun. Effects of continuous blood purification therapy on mitochondrial DNA and mitochondrial complex Ⅴ in peripheral blood mononuclear cells of sepsis patients[J]. Chinese Journal of General Practice, 2026, 24(2): 200-204. doi: 10.16766/j.cnki.issn.1674-4152.004360
shu

Effects of continuous blood purification therapy on mitochondrial DNA and mitochondrial complex Ⅴ in peripheral blood mononuclear cells of sepsis patients

doi: 10.16766/j.cnki.issn.1674-4152.004360

  • Department of Medical Intensive Care Unit, Shaoxing People ' s Hospital, Shaoxing, Zhejiang 312000, China

Funds:

 2023KY1261

  • Received Date: 2025-03-05
    Available Online: 2026-04-11
    Abstract
  •   Objective  To investigate the effects of continuous blood purification (CBP) therapy on mitochondrial DNA (mtDNA) and complex Ⅴ of monocytes in patients with sepsis, and to evaluate whether CBP therapy can improve mitochondrial function.  Methods  A prospective cohort study was conducted in 2023 to analyze the clinical data of 150 patients with sepsis admitted to the Department of Critical Care Medicine of Shaoxing People ' s Hospital from January 2023 to June 2024. Patients were divided into a CBP group (82 cases) and a non-CBP group (68 cases) according to whether the patients received CBP treatment. After 48 hours of treatment, the levels of mtDNA and mitochondrial complex Ⅴ in monocytes were detected in both groups. Twenty patients with similar age and disease severity were selected from each group. Monocytes were isolated 48 hours after treatment and culture in vitro. The levels of mtDNA, mitochondrial complex Ⅴ, TNF-α, and IL-6 were detected at 4, 8, 12, 24, and 48 hours after LPS stimulation.  Results  The mtDNA levels in both groups were significantly down-regulated compared with that in the group after 48 hours of treatment, and the mtDNA level in the CBP group was significantly lower than that in the non-CBP group (303.74±90.97 vs. 534.68±113.78, P < 0.01). The level of mitochondrial complex Ⅴ in the CBP group after 48-hour treatment was significantly higher than that in the control group [(101.12±33.46) pg/mL vs. (74.04±25.97) pg/mL, P < 0.01]. However, the complex Ⅴ levels were significantly decreased in the non-CBP group compared with the entry group [(57.54±13.89) pg/mL vs. (72.84±25.30) pg/mL, P < 0.01]. The levels of mtDNA, mitochondrial complex Ⅴ, TNF-α, and IL-6 of monocytes in the CBP group were significantly higher than those in the non-CBP group at 4 and 8h after incubation (P < 0.01).  Conclusion  CBP therapy may help improve monocyte mitochondrial function in patients with sepsis.

     

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