Volume 24 Issue 2
Feb.  2026
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Article Navigation >  Chinese Journal of General Practice  > 2026  >  24(2): 214-216
ZHUANG Ji, LI Zhen, HOU Yue, JIA Zhe, HU Yingxue, LI Yang, LIU Jie. Study on drug sensitivity and antigenic genotypes of Bordetella pertussis isolates in Chaoyang District, Beijing[J]. Chinese Journal of General Practice, 2026, 24(2): 214-216. doi: 10.16766/j.cnki.issn.1674-4152.004363
Citation: ZHUANG Ji, LI Zhen, HOU Yue, JIA Zhe, HU Yingxue, LI Yang, LIU Jie. Study on drug sensitivity and antigenic genotypes of Bordetella pertussis isolates in Chaoyang District, Beijing[J]. Chinese Journal of General Practice, 2026, 24(2): 214-216. doi: 10.16766/j.cnki.issn.1674-4152.004363
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Study on drug sensitivity and antigenic genotypes of Bordetella pertussis isolates in Chaoyang District, Beijing

doi: 10.16766/j.cnki.issn.1674-4152.004363
  • 1.

    Baotou Medical College, School of Public Health, Baotou, Inner Mongolia 014040, China

Funds:

 L202036

  • Received Date: 2025-02-09
    Available Online: 2026-04-11
    Abstract
  •   Objective  To collect nasopharyngeal swabs from suspected pertussis cases and isolate and culture Bordetella pertussis (Bp) for drug sensitivity and antigen genotype studies, thereby elucidating changes in drug sensitivity and antigen genotypes of Bp isolates in Chaoyang District, Beijing, from 2018 to 2023.  Methods  A total of 1 200 nasopharyngeal swab samples were collected from patients with suspected pertussis. Real-time fluorescent quantitative PCR was used for pathogen detection. Additionally, the E-test strip method was employed to evaluate the minimum inhibitory concentration (MIC) of Bp against 11 antimicrobial agents, including erythromycin and azithromycin. The genomic DNA of Bp isolates was extracted, and whole-genome sequencing was performed using a next-generation sequencing platform to obtain sequences of antigen genotypes and drug resistance-related loci.  Results  Sixty strains of Bp were successfully cultured. Epidemiological investigation revealed that 29 patients were under 1 year of age, accounting for 48.3%. Of the isolates, 59 strains exhibited resistance to erythromycin, clindamycin, azithromycin, and clarithromycin (MIC≥256 mg/L), while 1 strain was sensitive to azithromycin. The antigen genotypes of the 60 Bp strains were classified into two types: prn1/ptxP1/ptxA1/fim2-1/fim3-1/tcfA2 (5/60, 8.3%) and prn2/ptxP3/ptxA1/fim2-1/fim3-1/tcfA2 (55/60, 91.7%). All strains belonged to multi-locus sequence typing (MLST) type ST-2. A mutation of A2047G in the 23S rRNA gene was identified in all Bp strains.  Conclusion  The drug sensitivity and antigen genotype of Bp strains are undergoing notable changes, indicating the need for increased research on Bp strain variations.

     

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