Volume 24 Issue 2
Feb.  2026
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Article Navigation >  Chinese Journal of General Practice  > 2026  >  24(2): 280-284
GONG Chu, ZHAO Yiting, TANG Xinle, DU Minjie, ZHANG Yuxiang. Relationship between brain symptoms and the expression of intestinal flora metabolites and endoplasmic reticulum stress products in patients with mild carotid stenosis[J]. Chinese Journal of General Practice, 2026, 24(2): 280-284. doi: 10.16766/j.cnki.issn.1674-4152.004379
Citation: GONG Chu, ZHAO Yiting, TANG Xinle, DU Minjie, ZHANG Yuxiang. Relationship between brain symptoms and the expression of intestinal flora metabolites and endoplasmic reticulum stress products in patients with mild carotid stenosis[J]. Chinese Journal of General Practice, 2026, 24(2): 280-284. doi: 10.16766/j.cnki.issn.1674-4152.004379
shu

Relationship between brain symptoms and the expression of intestinal flora metabolites and endoplasmic reticulum stress products in patients with mild carotid stenosis

doi: 10.16766/j.cnki.issn.1674-4152.004379
  • 1.

    Department of Laboratory Medicine, Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830002, China

Funds:

 WIWY-202401

  • Received Date: 2025-09-11
    Available Online: 2026-04-11
    Abstract
  •   Objective  To explore the relationship between the occurrence of brain symptoms and the expression of intestinal flora metabolites and endoplasmic reticulum stress products in patients with mild carotid stenosis.  Methods  This study retrospectively analyzed the data sets of 87 patients who received carotid ultrasound examination in our hospital from January 2021 to June 2023, the mRNA level of the endoplasmic reticulum stress marker C/EBP homologous protein (CHOP) in peripheral blood was analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The fresh stool samples were collected for the first time after admission, and the levels of short-chain fatty acids (SCFAs) in the samples were analyzed by high-performance liquid chromatography.  Results  Plaque length, plaque area, and CHOP mRNA expression in peripheral blood in the symptomatic group increased significantly (P < 0.05), while the levels of intestinal flora metabolites such as acetic acid, propionic acid, butyric acid, isovaleric acid, and valeric acid decreased significantly (P < 0.05). CHOP mRNA expression was positively correlated with plaque length and plaque area (r=0.299 and 0.221, all P < 0.01), and negatively correlated with the levels of acetic acid, propionic acid, butyric acid, isovaleric acid, and valeric acid (r=-0.324, -0.285, -0.450, -0.233, and -0.332, all P < 0.05). The length of plaque was negatively correlated with butyric acid and isovaleric acid (r=-0.302 and -0.313, both P < 0.01). The plaque area was negatively correlated with propionic acid, butyric acid, and isovaleric acid (r=-0.230, -0.261, and -0.276, all P < 0.05). The prediction ability of butyric acid was the highest. When the cutoff value was 8.8, its AUC was 0.902, sensitivity was 77.1%, and specificity was 84.0%. Followed by CHOP mRNA expression, when the cutoff value was 0.60, its AUC was 0.811, sensitivity was 62.9%, and specificity was 94.0%.  Conclusion  The decrease of fecal acetic acid, propionic acid, butyric acid, isovaleric acid, and valeric acid levels and the increase of CHOP mRNA expression in peripheral blood may be related to the occurrence of brain symptoms, and these changes are related to the characteristics of carotid plaque.

     

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