Volume 24 Issue 3
Mar.  2026
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Article Navigation >  Chinese Journal of General Practice  > 2026  >  24(3): 407-410
XU Liya, CHENG Li, CHENG Xianying, WU Yanqin, LIU Minjie, YAN Guanghan, ZHANG Ting, XU Zhenyu. Effects and mechanism of exosomes derived from menstrual blood-derived stromal cells in restoring ovarian function in a rat model of premature ovarian failure[J]. Chinese Journal of General Practice, 2026, 24(3): 407-410. doi: 10.16766/j.cnki.issn.1674-4152.004407
Citation: XU Liya, CHENG Li, CHENG Xianying, WU Yanqin, LIU Minjie, YAN Guanghan, ZHANG Ting, XU Zhenyu. Effects and mechanism of exosomes derived from menstrual blood-derived stromal cells in restoring ovarian function in a rat model of premature ovarian failure[J]. Chinese Journal of General Practice, 2026, 24(3): 407-410. doi: 10.16766/j.cnki.issn.1674-4152.004407
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Effects and mechanism of exosomes derived from menstrual blood-derived stromal cells in restoring ovarian function in a rat model of premature ovarian failure

doi: 10.16766/j.cnki.issn.1674-4152.004407
  • 1.

    Department of Obstetrics and Gynecology, Shulan (Hangzhou) Hospital, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, Zhejiang 310022, China

Funds:

 2023ZL591

 20220020

 A202208

  • Received Date: 2025-02-10
    Available Online: 2026-06-02
    Abstract
  •   Objective  The purpose of this study was to explore the reparative effects and mechanisms of exosomes derived from menstrual blood-derived stromal cells (MenSCs-Exos) in restoring ovarian function of premature ovarian failure (POF) rats.  Methods  After the POF model was successfully established, it was divided into the transplantation treatment group and the placebo group (each 8 rats) using the random number table method. Both groups received tail vein injections of MenSCs-Exos and PBS. Control group (8 rats) was also set up. The physical conditions, body weight, and estrus cycle of the SD rats were monitored. Plasma was harvested to measure the levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), anti-Mullerian hormone (AMH), and estradiol (E2) by ELISA. The ovarian pathology and follicle counts were examined by HE. The protein concentration of B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in ovarian tissue were examined by ELISA.  Results  Rats in the transplantation group restored physical condition and the motility cycle after treatment. On day 14 after intervention, the weight of rats in the transplantation group was lower [(289.56±10.80) g] than that of the control group [(313.38±8.94) g] and higher than that of the placebo group [(280.82±4.40) g, P < 0.05]. The levels of LH and FSH in the transplantation group were significantly lower than those in the placebo group and higher than those in the control group, while the levels of AMH and E2 were significantly higher than those in the placebo group and lower than those in the control group (P < 0.05). The pathological morphology of the ovaries in the transplantation group was improved, with follicle numbers at all developmental stages lower than those of the control group and higher than those of the placebo group (P < 0.05). In addition, the concentration of Bcl-2 protein was lower than that of the control group and higher than that of the placebo group, while the concentration of Bax protein was higher than that of the control group and lower than that of the placebo group (P < 0.05).  Conclusion  MenSCs-Exos transplantation effectively restores ovarian function and ameliorates ovarian damage, potentially through regulation of apoptosis-related protein expression.

     

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