Determination of neobavaisoflavone in Psoralea corylifolia L. by HPLC

Objective To establish a high performance liquid chromatography (HPLC) method for the content determination of neobavaisoflavone in Psoralea corylifolia L. Select the suitable extraction solvent,suitable mobile phase, appropriate determination wavelength and determine the optimal ultrasonic extraction time extraction of Psoralea corylifolia L. of neobavaisoflavone. Methods HPLC was applied and the determination was performed on WondaSil^® C₁₈(150.0 mm×4.6 mm,5 μm).0.05% trifluoroacetic acid aqueous solution as the mobile phase A,and the acetonitrile as mobile phase B,gradient elution HPLC method was applied,solution at a flow rate of 1.0 ml/min and detection wavelength at 254 nm.The column temperature was 30℃,and injection volume was 20 μl.100% 30 min methanol ultrasonic extraction of neobavaisoflavone.The precision of the control sample was repeated 6 times with the same concentration of the control sample.Parallel system repeatability 6 prepared neobavaisoflavone sample solution into the sample solution.The stability of the same neobavaisoflavone sample solution was detemined at different time sampling 5 times the sample solution.Parallel preparation of 6 samples of the sample solution to add the same amount of control samples calculated sample recovery rate. Results Through methodological study, the linear regression equation of neobavaisoflavone is:A=79 375C-9 063.7,R²=0.999,the linear range of Neobavaisoflavone was 0.75-12.00 μg/ml,neobavaisoflavone concentration and peak area showed a good linear relationship.Sample average recovery rate was 97.51%,RSD=1.21%(n=6).Precision,repeatability,stability and recovery were all good.There were differences in neobavaisoflavone different sources of Psoralea corylifolia L. Conclusion By optimizing the chromatographic condition,this paper establishes the method for the determination of Psoralea corylifolia L. in neobavaisoflavone content, examines the special attributes,standard curve,limit of detection and quantitative limit,precision,repetition,stability,and recovery rate.The results showed that the HPLC method for the content determination of neobavaisoflavone in Psoralea corylifolia Lis user-friendly,accurate and reliable, with good repeatability and stability,which can be used for quality control of Psoralea corylifolia L.