Effects of miR-34a on proliferation and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by targeting FOXM1

GE Si-chen; JIANG Cheng-yi

doi:10.16766/j.cnki.issn.1674-4152.002223

Volume 19 Issue 12

Dec. 2021

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Article Navigation > Chinese Journal of General Practice > 2021 > 19(12): 2012-2015,2023

GE Si-chen, JIANG Cheng-yi. Effects of miR-34a on proliferation and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by targeting FOXM1[J]. Chinese Journal of General Practice, 2021, 19(12): 2012-2015,2023. doi: 10.16766/j.cnki.issn.1674-4152.002223

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GE Si-chen, JIANG Cheng-yi. Effects of miR-34a on proliferation and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by targeting FOXM1[J]. Chinese Journal of General Practice, 2021, 19(12): 2012-2015,2023. doi: 10.16766/j.cnki.issn.1674-4152.002223

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Effects of miR-34a on proliferation and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by targeting FOXM1

doi: 10.16766/j.cnki.issn.1674-4152.002223

GE Si-chen
, JIANG Cheng-yi^,

Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

Funds:

KJ20180996

Received Date: 2020-11-08
Available Online: 2022-03-02

Abstract

Abstract

Objective To investigate the effect of miR-34a on the proliferation, invasion and migration of nasopharyngeal carcinoma cells by targeting forkhead box protein M1 (FOXM1) and the mechanism of invasion and migration from the perspective of epithelial-mesenchymal transformation. Methods Human nasopharyngeal carcinoma cells (HNE-1 and CNE-2Z) were cultured in vitro. Each cell line was divided into three groups, namely the blank control group (control group), the negative control group (miR-34a nc group) and the overexpression of miR-34a group (miR-34a mimics group). The expression level of miR-34a in each group was detected by qRT-PCR after transfection. The targeted relationship between miR-34a and FOXM1 was predicted by bioinformatics software and then analysed by qRT-PCR and western blot. After overexpression of miR-34a, cell proliferation was detected by CCK-8 assay, cell migration and invasion were detected by Transwell test, and the relative expression levels of invasion- and migration-related proteins such as E-cadherin and Vimentin were detected by western blot. Results The possible targeting relationship between miR-34a and FOXM1 was predicted using bioinformatics software. In HNE-1 and CNE-2Z cells, compared with the control group and miR-34a nc group, the expression level of FOXM1 was down-regulated in the miR-34a mimics group (HNE-1: 0.570±0.041, 1.127±0.129, 1.125±0.145, F=23.672, P=0.001; CNE-2Z: 0.689±0.114, 1.966±0.164, 1.924±0.087, F=99.599, P < 0.001); proliferation, migration and invasion abilities were significantly increased (P < 0.01); E-cadherin expression was increased, and Vimentin expression was decreased (P < 0.01). No statistically significant difference was observed between the control group and miR-34a nc group (P>0.05). Conclusion miR-34a promotes the proliferation, invasion and migration of nasopharyngeal carcinoma cells by targeting FOXM1, which is associated with the inhibition of epithelial-mesenchymal transformation.
- MiR-34a,
- Forkhead box protein M1,
- Nasopharyngeal carcinoma,
- Cell proliferation,
- Epithelial-mesenchymal transition

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References

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Effects of miR-34a on proliferation and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by targeting FOXM1

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Effects of miR-34a on proliferation and epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by targeting FOXM1

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