Volume 20 Issue 5
May  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(5): 761-765
ZHANG Chang-yin, TAO Zhong-jie, ZHU Chen-jie. Effect of salidroside on the proliferation and apoptosis of testicular spermatogenic cells in infertile rats[J]. Chinese Journal of General Practice, 2022, 20(5): 761-765. doi: 10.16766/j.cnki.issn.1674-4152.002448
Citation: ZHANG Chang-yin, TAO Zhong-jie, ZHU Chen-jie. Effect of salidroside on the proliferation and apoptosis of testicular spermatogenic cells in infertile rats[J]. Chinese Journal of General Practice, 2022, 20(5): 761-765. doi: 10.16766/j.cnki.issn.1674-4152.002448
shu

Effect of salidroside on the proliferation and apoptosis of testicular spermatogenic cells in infertile rats

doi: 10.16766/j.cnki.issn.1674-4152.002448

  • Department of Urology, Pinghu Hospital of Traditional Chinese Medicine, Pinghu, Zhejiang 314200, China

Funds:

 2019ZB126

  • Received Date: 2021-05-04
    Available Online: 2022-09-05
    Abstract
  •   Objective  To investigate the effect of salidroside on the proliferation and apoptosis of spermatogenic cells in infertile rats.  Methods  Fifteen rats were Selected from 60 rats as group A, and 45 infertility model rats were randomly divided into groups B, C and D, with 15 rats in each group. Groups C and D were given 50 and 100 mg/kg of salidroside via gavage, and groups A and B were given the same amount of normal saline. Serum testosterone, follicle-stimulating hormone, and luteinising hormone were detected. Superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by enzyme-linked immunosorbent assay. Haematoxylin and eosin (HE) staining was used to detect the morphology of spermatogenic cells. Proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry, and the apoptosis rate of spermatogenic cells was detected by TUNEL assay. Western blotting was used to detect the relative expression of fatty acid synthase (Fas), fatty acid synthase ligand (Fasl) and caspase-3.  Results  Compared with that in group A, the SOD levels in groups B, C and D were decreased, and the order was follows: group B < group C < group D (all P < 0.05). Compared with those in group A, the relative expression levels of MDA, Fas, Fasl, and caspase-3 protein in groups B, C and D were increased, and the order was as follows: group B>group C>group D (all P < 0.05). Compared with group B, the structure of testicular cells, atrophy of seminiferous tubules, and exfoliation of spermatogenic cells in group D were significantly improved. The percentage of PCNA in spermatogenic cells of group A was (41.23±5.46)%, which was higher than that in group B (20.13±3.21)%, group C [(27.64±3.25)%] and group D [(35.11±4.56)%], all P < 0.05. The apoptosis rate of spermatogenic cells in group A was (2.23±0.03)%, which was lower than that in group B [(10.03±1.12)%], group C [(7.94±0.89)%] and group D [(4.07±0.52)%], all P < 0.05.  Conclusion  Salidroside can regulate the proliferation and apoptosis of spermatogenic cells in infertile rats. The mechanism may be related to the downregulation of the Fas/Fasl signalling pathway.

     

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