Volume 20 Issue 5
May  2022
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Article Navigation >  Chinese Journal of General Practice  > 2022  >  20(5): 785-788
ZHANG Gui-bing, HE Zheng-fei, SHANG Wen-zhong, WANG Ai-wei, WU Yan-fang, HUANG Hui-yan. Effect of triethanolamine on the biological characteristics of diffuse large B-cell lymphoma cells[J]. Chinese Journal of General Practice, 2022, 20(5): 785-788. doi: 10.16766/j.cnki.issn.1674-4152.002454
Citation: ZHANG Gui-bing, HE Zheng-fei, SHANG Wen-zhong, WANG Ai-wei, WU Yan-fang, HUANG Hui-yan. Effect of triethanolamine on the biological characteristics of diffuse large B-cell lymphoma cells[J]. Chinese Journal of General Practice, 2022, 20(5): 785-788. doi: 10.16766/j.cnki.issn.1674-4152.002454
shu

Effect of triethanolamine on the biological characteristics of diffuse large B-cell lymphoma cells

doi: 10.16766/j.cnki.issn.1674-4152.002454

  • Department of Hematdogy, the First People's Hospital of Fuyang District, Hangzhou City, Hangzhou, Zhejiang 311400, China

Funds:

 2020ZA098

  • Received Date: 2021-05-28
    Available Online: 2022-09-05
    Abstract
  •   Objective  To observe the effect of triethanolamine (TEOA) on the biological characteristics of diffuse large B-cell lymphoma (DLBCL) cells, so as to provide a basis for clinical treatment of the disease.  Methods  DLBCL cell lines OCI-LY3 were provided by the cell bank of the Chinese Academy of Sciences. They were randomised into the control group and observation group by random number table method, with five parallel holes in each group. Different concentrations (5, 10, 15, 20, 25, 30, 35, 40, 45, 50 μmol/L) of TEOA were added into the observation group and then cultured for 12 h. The GraphPad Prism 5 software was used to calculate the survival rates of DLBCL cells in both groups, flow cytometry was used to detect the apoptosis and invasion of DLBCL cells, the MTT method was used to detect the proliferation of DLBCL cells, and Western Blotting was used to detect the relative protein expression. The levels of reactive oxygen species (ROS) in both groups were compared, and research data were collected and input into the SPSS 20.0 statistical software for statistical analysis.  Results  After 24, 48 and 72 h of TEOA treatment, the cell proliferation rates in the observation group[(39.24±3.95)%, (57.08±5.87)% and (114.08±14.69)%] were significantly lower than those in the control group[(49.49±5.02)%, (95.12±9.64)% and (210.45±25.62)%, all P < 0.05]. The observation group had significantly smaller number of transmembrane cells (38.14±3.77) and significantly higher apoptosis rate[(12.14±1.24)%] than the control group[92.25±9.24 and (3.64±0.36)%, respectively, all P < 0.05]. TEOA could increase ROS levels in DLBCL OCI-LY3 cells and effectively upregulate the expression levels of p38, p-Chk1, p-Chk2 and γ-H2AX in OCI-LY3.  Conclusion  TEOA can inhibit cell proliferation and invasion whilst effectively inducing cell apoptosis and ROS expression. TEOA acting on DLBCL OCI-LY3 cells can promote the expression of p38, p-Chk1, p-Chk2 and γ-H2AX and induce the activation of the p38 MAPK pathway.

     

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