UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis

HE Xuxu; ZHAO Meng; YANG Yiqun; ZUO Lugen; GENG Zhijun; GE Sitang; CHEN Deli

doi:10.16766/j.cnki.issn.1674-4152.002927

Volume 21 Issue 4

Apr. 2023

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Article Navigation > Chinese Journal of General Practice > 2023 > 21(4): 544-548

HE Xuxu, ZHAO Meng, YANG Yiqun, ZUO Lugen, GENG Zhijun, GE Sitang, CHEN Deli. UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis[J]. Chinese Journal of General Practice, 2023, 21(4): 544-548. doi: 10.16766/j.cnki.issn.1674-4152.002927

Citation:

HE Xuxu, ZHAO Meng, YANG Yiqun, ZUO Lugen, GENG Zhijun, GE Sitang, CHEN Deli. UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis[J]. Chinese Journal of General Practice, 2023, 21(4): 544-548. doi: 10.16766/j.cnki.issn.1674-4152.002927

Citation:

HE Xuxu, ZHAO Meng, YANG Yiqun, ZUO Lugen, GENG Zhijun, GE Sitang, CHEN Deli. UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis[J]. Chinese Journal of General Practice, 2023, 21(4): 544-548. doi: 10.16766/j.cnki.issn.1674-4152.002927

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UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis

doi: 10.16766/j.cnki.issn.1674-4152.002927

HE Xuxu^{1, 2}
, ZHAO Meng^{1, 2}
, YANG Yiqun^{1, 2}
, ZUO Lugen²
, GENG Zhijun³
, GE Sitang²
, CHEN Deli^{2
,
,}

1.
Graduate School of Bengbu Medical College, Bengbu, Anhui 233030, China

Funds:

KJ2021A0685

Received Date: 2022-09-05
Available Online: 2023-05-31

Abstract

Abstract

Objective To study the expression of uridine diphosphate-galactose glycosyltransferase 8(UGT8) in gastric cancer and its association with long-term prognosis and its effect on the metastatic ability of gastric cancer. Methods A total of 109 patients who underwent radical gastric cancer resection in the First Affiliated Hospital of Bengbu Medical College from December 2013 to October 2016 were included in this study.The expression level of UGT8 in gastric cancer tissues was detected by immunohistochemistry and western blotting.Statistical methods were applied to analyze the relationship between UGT8 level and clinicopathological parameters in gastric cancer.The Cox regression and ROC curve were used to evaluate the independent risk factors affecting the 5-year survival rate and the diagnostic efficacy of UGT8.The GEO database was used to obtain a dataset of gastric cancer, and GO and KEGG enrichment analysis was performed on UGT8.The effect of UGT8 on metastasis of gastric cancer cells (MGC-803) was analyzed in vitro. Results The expression level of UGT8 in gastric cancer tissues was significantly higher than that in adjacent tissues (P < 0.01);The proportion of patients with increased tumor diameter (≥5 cm), T3-4 stage, N2-3 stage, serum CEA≥5 μg/L and serum CA19-9≥37 kU/L in the UGT8 high expression group was significantly higher than that in the UGT8 low expression group (all P < 0.05).Cox regression analysis showed that high UGT8 expression was an independent risk factor affecting the 5-year survival rate of gastric cancer patients.ROC curve analysis showed that UGT8 had a sensitivity of 73.00% and a specificity of 78.30% for predicting death at 5 years after surgery in patients with gastric cancer (P < 0.01).GO enrichment analysis showed that UGT8 was involved in cadherin binding activity, and KEGG enrichment results showed that UGT8 was mainly involved in adhesion pathways.Western blotting showed that UGT8 promoted the phosphorylation level of SDF-1 and CXCR4 in metastasis-related signals (P < 0.05). Conclusion The high expression of UGT8 in gastric cancer tissues is associated with poor prognosis, and may be involved in the metastasis of gastric cancer cells by regulating SDF-1/CXCR4 signaling pathway.
- Gastric cancer,
- Uridine diphosphate-galactose glycosyltransferase 8 (UGT8),
- Long-term prognosis,
- Metastasis,
- Biomarkers

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References(21)

References

[1]	ARNOLD M, PARK J Y, CAMARGO M C, et al. Is gastric cancer becoming a rare disease? A global assessment of predicted incidence trends to 2035[J]. Gut, 2020, 69(5): 823-829. doi: 10.1136/gutjnl-2019-320234
[2]	HEER E V, HARPER A S, SUNG H, et al. Emerging cancer incidence trends in Canada: The growing burden of young adult cancers[J]. Cancer, 2020, 126(20): 4553-4562. doi: 10.1002/cncr.33050
[3]	曹毛毛, 李贺, 孙殿钦, 等. 2000—2019年中国胃癌流行病学趋势分析[J]. 中华消化外科杂志, 2021, 20(1): 102-109. CAO M M, LI H, SUN D Q, et al. Epidemiological trend analysis of gastric cancer in China from 2000 to 201[J]. Chinese Journal of Digestive Surgery, 2021, 20(1): 102-109.
[4]	MEECH R, HU D G, MCKINNON R A, et al. The UDP-Glycosyltransferase (UGT) superfamily: new members, new functions, and novel paradigms[J]. Physiol Rev, 2019, 99(2): 1153-1222. doi: 10.1152/physrev.00058.2017
[5]	JI J, XIE M R, QIAN Q L, et al. SOX9-mediated UGT8 expression promotes glycolysis and maintains the malignancy of non-small cell lung cancer[J]. Biochem Biophys Res Commun, 2022, 587: 139-145. doi: 10.1016/j.bbrc.2021.11.099
[6]	DZIEGIEL P, OWCZAREK T, PLAZUK E, et al. Ceramide galactosyltransferase (UGT8) is a molecular marker of breast cancer malignancy and lung metastases[J]. Br J Cancer, 2010, 103(4): 524-531. doi: 10.1038/sj.bjc.6605750
[7]	RZECHONEK A, CYGAN M, BLASIAK P, et al. Expression of ceramide galactosyltransferase (UGT8) in primary and metastatic lung tissues of non-small-cell lung cancer[J]. Adv Exp Med Biol, 2016, 952: 51-58. http://www.researchgate.net/profile/Vladimir_Bobek/publication/308039318_Expression_of_Ceramide_Galactosyltransferase_UGT8_in_Primary_and_Metastatic_Lung_Tissues_of_Non-Small-Cell_Lung_Cancer/links/57ebf38308ae92a5dbd0564b.pdf
[8]	CAO Q, CHEN X, WU X, et al. Inhibition of UGT8 suppresses basal-like breast cancer progression by attenuating sulfatide-αVβ5 axis[J]. J Exp Med, 2018, 215(6): 1679-1692. doi: 10.1084/jem.20172048
[9]	LYDEN D, GHAJAR C M, CORREIA A L, et al. Metastasis[J]. Cancer Cell, 2022, 40(8): 787-791. doi: 10.1016/j.ccell.2022.07.010
[10]	WEISS F, LAUFFENBURGER D, FRIEDL P. Towards targeting of shared mechanisms of cancer metastasis and therapy resistance[J]. Nat Rev Cancer, 2022, 22(3): 157-173. doi: 10.1038/s41568-021-00427-0
[11]	WANG Y, YAN B, NI L, et al. The clinical significance and functional role of miR-466 in gastric cancer peritoneal metastasis[J]. Mol Biotechnol, 2022, 64(1): 25-32. doi: 10.1007/s12033-021-00382-z
[12]	BUSSO-LOPES A F, NEVES L X, CAMARA G A, et al. Connecting multiple microenvironment proteomes uncovers the biology in head and neck cancer[J]. Nat Commun, 2022, 13(1): 6725. doi: 10.1038/s41467-022-34407-1
[13]	WANG P, WANG Z, LIU J. Role of HDACs in normal and malignant hematopoiesis[J]. Mol Cancer, 2020, 19(1): 5. doi: 10.1186/s12943-019-1127-7
[14]	OLKKONEN V M. The emerging roles of OSBP-related proteins in cancer: impacts through phosphoinositide metabolism and protein-protein interactions[J]. Biochem Pharmacol, 2022, 196: 114455. DOI: 10.1016/j.bcp.2021.114455.
[15]	CHEN I X, CHAUHAN V P, POSADA J, et al. Blocking CXCR4 alleviates desmoplasia, increases T-lymphocyte infiltration, and improves immunotherapy in metastatic breast cancer[J]. Proc Natl Acad Sci U S A, 2019, 116(10): 4558-4566. doi: 10.1073/pnas.1815515116
[16]	WANG R X, JI P, GONG Y, et al. SDF-1 expression and tumor-infiltrating lymphocytes identify clinical subtypes of triple-negative breast cancer with different responses to neoadjuvant chemotherapy and survival[J]. Front Immunol, 2022, 13: 940635. DOI: 10.3389/fimmu.2022.940635.
[17]	LEE K C, WU K L, CHANG S F, et al. Fermented ginger extract in natural deep eutectic solvent enhances cytotoxicity by inhibiting NF-κB mediated CXC chemokine receptor 4 expression in oxaliplatin-resistant human colorectal Cancer Cells[J]. Antioxidants (Basel), 2022, 11(10): 2057. doi: 10.3390/antiox11102057
[18]	ZHOU W Q, GUO S C, LIU M L, et al. Targeting CXCL12/CXCR4 axis in tumor immunotherapy[J]. Curr Med Chem, 2019, 26(17): 3026-3041. doi: 10.2174/0929867324666170830111531
[19]	LEO M, SABATINO L. Targeting CXCR4 and CD47 receptors: an overview of new and old molecules for a biological personalized anticancer Therapy[J]. Int J Mol Sci, 2022, 23(20): 12499. DOI: 10.3390/ijms232012499.
[20]	陈琛, 李胜泽. SDF-1/CXCR4生物学轴在宫颈癌中的研究进展[J]. 中华全科医学, 2014, 12(3): 451-453. https://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201403052.htm CHEN C, LI S Z. Research Progress of SDF-1/CXCR4 Axis in Cervical Cancer[J]. Chinese Journal of General Practice, 2014, 12(3): 451-453. https://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201403052.htm
[21]	ALSAYED R, KHAN A Q, AHMAD F, et al. Epigenetic regulation of CXCR4 signaling in cancer pathogenesis and progression[J]. Semin Cancer Biol, 2022, 86(Pt 2): 697-708. http://pubmed.ncbi.nlm.nih.gov/35346802/

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UGT8 expression in gastric cancer tissues and its regulatory effect on gastric cancer metastasis

doi: 10.16766/j.cnki.issn.1674-4152.002927